Mutational Analysis of Measles Virus Suggests Constraints on Antigenic Variation of the Glycoproteins.

Published

Journal Article

Measles virus undergoes error-prone replication like other RNA viruses, but over time, it has remained antigenically monotypic. The constraints on the virus that prevent the emergence of antigenic variants are unclear. As a first step in understanding this question, we subjected the measles virus genome to unbiased insertional mutagenesis, and viruses that could tolerate insertions were rescued. Only insertions in the nucleoprotein, phosphoprotein, matrix protein, as well as intergenic regions were easily recoverable. Insertions in the glycoproteins of measles virus were severely under-represented in our screen. Host immunity depends on developing neutralizing antibodies to the hemagglutinin and fusion glycoproteins; our analysis suggests that these proteins occupy very little evolutionary space and therefore have difficulty changing in the face of selective pressures. We propose that the inelasticity of these proteins prevents the sequence variation required to escape antibody neutralization in the host, allowing for long-lived immunity after infection with the virus.

Full Text

Duke Authors

Cited Authors

  • Fulton, BO; Sachs, D; Beaty, SM; Won, ST; Lee, B; Palese, P; Heaton, NS

Published Date

  • June 2015

Published In

Volume / Issue

  • 11 / 9

Start / End Page

  • 1331 - 1338

PubMed ID

  • 26004185

Pubmed Central ID

  • 26004185

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

International Standard Serial Number (ISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2015.04.054

Language

  • eng