Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins.

Journal Article (Journal Article)

The molecular basis for the diversity across influenza strains is poorly understood. To gain insight into this question, we mutagenized the viral genome and sequenced recoverable viruses. Only two small regions in the genome were enriched for insertions, the hemagglutinin head and the immune-modulatory nonstructural protein 1. These proteins play a major role in host adaptation, and thus need to be able to evolve rapidly. We propose a model in which certain influenza A virus proteins (or protein domains) exist as highly plastic scaffolds, which will readily accept mutations yet retain their functionality. This model implies that the ability to rapidly acquire mutations is an inherent aspect of influenza HA and nonstructural protein 1 proteins; further, this may explain why rapid antigenic drift and a broad host range is observed with influenza A virus and not with some other RNA viruses.

Full Text

Duke Authors

Cited Authors

  • Heaton, NS; Sachs, D; Chen, C-J; Hai, R; Palese, P

Published Date

  • December 10, 2013

Published In

Volume / Issue

  • 110 / 50

Start / End Page

  • 20248 - 20253

PubMed ID

  • 24277853

Pubmed Central ID

  • PMC3864309

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1320524110


  • eng

Conference Location

  • United States