High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor.

Journal Article

Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of ∼ 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.

Full Text

Duke Authors

Cited Authors

  • St-Pierre, F; Marshall, JD; Yang, Y; Gong, Y; Schnitzer, MJ; Lin, MZ

Published Date

  • June 2014

Published In

Volume / Issue

  • 17 / 6

Start / End Page

  • 884 - 889

PubMed ID

  • 24755780

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

International Standard Serial Number (ISSN)

  • 1097-6256

Digital Object Identifier (DOI)

  • 10.1038/nn.3709

Language

  • eng