Development of microsatellite markers for the apomictic triploid fern Myriopteris lindheimeri (Pteridaceae).

Journal Article

Microsatellite markers were developed for investigating the population dynamics of Myriopteris lindheimeri (Pteridaceae), an apomictic triploid fern endemic to deserts of the southwestern United States and Mexico.Using 454 sequencing, 21 microsatellite markers were developed. Of these, 14 were polymorphic with up to five alleles per locus and eight markers amplified in one or more congeneric close relatives (M. covillei, M. fendleri, M. aurea, and M. rufa). To demonstrate marker utility, M. lindheimeri samples from three Arizona populations were genotyped at nine loci. For each population, diversity measures including percent polymorphic loci, frequency of heterozygotes across all loci, and genotypic diversity were calculated. Across the three populations, on average, 63% of loci were polymorphic, the average frequency of heterozygotes (across all loci) was 0.32, and average genotypic diversity was 0.34.These markers provide a foundation for future studies exploring polyploidy and apomixis in myriopterid ferns.

Full Text

Duke Authors

Cited Authors

  • Grusz, AL; Pryer, KM

Published Date

  • November 10, 2015

Published In

Volume / Issue

  • 3 / 11

PubMed ID

  • 26649266

Electronic International Standard Serial Number (EISSN)

  • 2168-0450

International Standard Serial Number (ISSN)

  • 2168-0450

Digital Object Identifier (DOI)

  • 10.3732/apps.1500061

Language

  • eng