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Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature.

Publication ,  Journal Article
Wang, T; Ge, Y; Xiao, M; Lopez-Coral, A; Azuma, R; Somasundaram, R; Zhang, G; Wei, Z; Xu, X; Rauscher, FJ; Herlyn, M; Kaufman, RE
Published in: Pigment Cell Melanoma Res
July 2012

The presence of tumor-associated macrophages (TAMs) in melanomas is correlated with a poor clinical prognosis. However, there is limited information on the characteristics and biological activities of human TAMs in melanomas. In this study, we developed an in vitro method to differentiate human monocytes to macrophages using modified melanoma-conditioned medium (MCM). We demonstrate that factors from MCM-induced macrophages (MCMI-Mφ) express both M1-Mφ and M2-Mφ markers and inhibit melanoma-specific T-cell proliferation. Furthermore, microarray analyses reveal that the majority of genes up-regulated in MCMI-Mφ are associated with tumor invasion. The most strikingly up-regulated genes are CCL2 and MMP-9. Consistent with this, blockade of both CCL-2 and MMPs diminish MCMI-Mφ-induced melanoma invasion. Finally, we demonstrated that both MCMI-Mφ and in vivo TAMs express the pro-invasive, melanoma-associated gene, glycoprotein non-metastatic melanoma protein B. Our study provides a framework for understanding the mechanisms of cross-talk between TAMs and melanoma cells within the tumor microenvironment.

Duke Scholars

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Published In

Pigment Cell Melanoma Res

DOI

EISSN

1755-148X

Publication Date

July 2012

Volume

25

Issue

4

Start / End Page

493 / 505

Location

England

Related Subject Headings

  • Skin Neoplasms
  • Neoplasm Invasiveness
  • Monocytes
  • Melanoma
  • Matrix Metalloproteinase 9
  • Macrophages
  • Macrophage Colony-Stimulating Factor
  • Humans
  • Genes, Neoplasm
  • Gene Expression Regulation, Neoplastic
 

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Wang, T., Ge, Y., Xiao, M., Lopez-Coral, A., Azuma, R., Somasundaram, R., … Kaufman, R. E. (2012). Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature. Pigment Cell Melanoma Res, 25(4), 493–505. https://doi.org/10.1111/j.1755-148X.2012.01005.x
Wang, Tao, Yingbin Ge, Min Xiao, Alfonso Lopez-Coral, Rikka Azuma, Rajasekharan Somasundaram, Gao Zhang, et al. “Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature.Pigment Cell Melanoma Res 25, no. 4 (July 2012): 493–505. https://doi.org/10.1111/j.1755-148X.2012.01005.x.
Wang T, Ge Y, Xiao M, Lopez-Coral A, Azuma R, Somasundaram R, et al. Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature. Pigment Cell Melanoma Res. 2012 Jul;25(4):493–505.
Wang, Tao, et al. “Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature.Pigment Cell Melanoma Res, vol. 25, no. 4, July 2012, pp. 493–505. Pubmed, doi:10.1111/j.1755-148X.2012.01005.x.
Wang T, Ge Y, Xiao M, Lopez-Coral A, Azuma R, Somasundaram R, Zhang G, Wei Z, Xu X, Rauscher FJ, Herlyn M, Kaufman RE. Melanoma-derived conditioned media efficiently induce the differentiation of monocytes to macrophages that display a highly invasive gene signature. Pigment Cell Melanoma Res. 2012 Jul;25(4):493–505.
Journal cover image

Published In

Pigment Cell Melanoma Res

DOI

EISSN

1755-148X

Publication Date

July 2012

Volume

25

Issue

4

Start / End Page

493 / 505

Location

England

Related Subject Headings

  • Skin Neoplasms
  • Neoplasm Invasiveness
  • Monocytes
  • Melanoma
  • Matrix Metalloproteinase 9
  • Macrophages
  • Macrophage Colony-Stimulating Factor
  • Humans
  • Genes, Neoplasm
  • Gene Expression Regulation, Neoplastic