Absence of TGF-beta receptors and growth inhibitory responses in retinoblastoma cells.

Journal Article

The responses of retinoblastoma tumor cells and normal retinal cells to various growth inhibitory factors were examined. Whereas fetal retinal cells were highly sensitive to the antimitogenic effects of transforming growth factor beta 1 (TGF-beta 1), retinoblastoma tumor cell lines were all resistant to this factor. Binding assays and affinity labeling of these cells with radioiodinated TGF-beta 1 revealed that the cells did not have TGF-beta receptors. The retinoblastoma cells lacked the three affinity-labeled proteins of 65, 95, and 300 kilodaltons typically seen in human cell lines and thus differed from normal retinal cells and from other types of neuroectodermal tumors that display the normal pattern of receptors. Loss of TGF-beta receptors, which is a rare event among tumor cells, may represent one mechanism through which these cells escape from negative control and form retinoblastomas.

Full Text

Duke Authors

Cited Authors

  • Kimchi, A; Wang, XF; Weinberg, RA; Cheifetz, S; Massagué, J

Published Date

  • April 1988

Published In

Volume / Issue

  • 240 / 4849

Start / End Page

  • 196 - 199

PubMed ID

  • 2895499

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.2895499

Language

  • eng