Spatial frequency-domain multiplexed microscopy for simultaneous, single-camera, one-shot, fluorescent, and quantitative-phase imaging.


Journal Article

Multimodal imaging is a crucial tool when imaging biological phenomena that cannot be comprehensively captured by a single modality. Here, we introduce a theoretical framework for spatial-frequency-multiplexed microscopy via off-axis interference as a novel wide-field imaging technique that enables true simultaneous multimodal and multichannel wide-field imaging. We experimentally demonstrate this technique for single-camera, simultaneous two-channel fluorescence and one-channel quantitative-phase imaging for fluorescent microspheres and fixed cells stained for F-actin and nuclear fluorescence.

Full Text

Duke Authors

Cited Authors

  • Chowdhury, S; Eldridge, WJ; Wax, A; Izatt, JA

Published Date

  • November 2015

Published In

Volume / Issue

  • 40 / 21

Start / End Page

  • 4839 - 4842

PubMed ID

  • 26512463

Pubmed Central ID

  • 26512463

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.40.004839


  • eng