Role of Polarized G Protein Signaling in Tracking Pheromone Gradients.

Published

Journal Article

Yeast cells track gradients of pheromones to locate mating partners. Intuition suggests that uniform distribution of pheromone receptors over the cell surface would yield optimal gradient sensing. However, yeast cells display polarized receptors. The benefit of such polarization was unknown. During gradient tracking, cell growth is directed by a patch of polarity regulators that wanders around the cortex. Patch movement is sensitive to pheromone dose, with wandering reduced on the up-gradient side of the cell, resulting in net growth in that direction. Mathematical modeling suggests that active receptors and associated G proteins lag behind the polarity patch and act as an effective drag on patch movement. In vivo, the polarity patch is trailed by a G protein-rich domain, and this polarized distribution of G proteins is required to constrain patch wandering. Our findings explain why G protein polarization is beneficial and illuminate a novel mechanism for gradient tracking.

Full Text

Duke Authors

Cited Authors

  • McClure, AW; Minakova, M; Dyer, JM; Zyla, TR; Elston, TC; Lew, DJ

Published Date

  • November 2015

Published In

Volume / Issue

  • 35 / 4

Start / End Page

  • 471 - 482

PubMed ID

  • 26609960

Pubmed Central ID

  • 26609960

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2015.10.024

Language

  • eng