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High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma.

Publication ,  Journal Article
Peng, L; Liang, J; Wang, H; Song, X; Rashid, A; Gomez, HF; Corley, LJ; Abbruzzese, JL; Fleming, JB; Evans, DB; Wang, H
Published in: Clin Cancer Res
July 15, 2010

PURPOSE: Nucleolin is a major nucleolar protein that has been shown to be overexpressed in rapidly dividing cells and plays an essential role in cell proliferation and survival. However, the expression and significance of nucleolin in pancreatic ductal adenocarcinoma (PDA) have not been studied. EXPERIMENTAL DESIGN: We used a tissue microarray consisting of 1.0-mm cores of tumor and paired nonneoplastic pancreatic tissue from 69 pancreaticoduodenectomy specimens with stage II PDA. Nucleolin expression was evaluated by immunohistochemistry and scored quantitatively by image analysis. Nucleolin expression was classified as nucleolin-high or nucleolin-low using the median nucleolin labeling index of 3.5% as cutoff. Staining results were correlated with clinicopathologic features and survival. RESULTS: Both PDAs and PDA cell lines showed nucleolar staining for nucleolin. Nucleolin expression was higher in PDAs and PDA cell lines than in nonneoplastic ductal epithelial cells. Among the 69 stage II PDAs, 34 (49%) were nucleolin-high. The median overall survival was 65.2 +/- 16.3 months for patients who had nucleolin-high PDAs compared with 19.5 +/- 3.3 months for patients whose tumors were nucleolin-low (P = 0.03, log-rank method). No significant correlation between nucleolin expression and other clinicopathologic parameters was found. In multivariate analysis, nucleolin expression was a prognostic factor for overall survival in patients with stage II PDA independent of patient's age, gender, tumor size, differentiation, and lymph node status. CONCLUSIONS: Nucleolin was overexpressed in PDAs and PDA cell lines. A high level of nucleolar expression of nucleolin was an independent prognostic marker for better survival for patients with stage II PDAs.

Duke Scholars

Published In

Clin Cancer Res

DOI

EISSN

1557-3265

Publication Date

July 15, 2010

Volume

16

Issue

14

Start / End Page

3734 / 3742

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tissue Array Analysis
  • Retrospective Studies
  • RNA-Binding Proteins
  • Prognosis
  • Phosphoproteins
  • Pancreatic Neoplasms
  • Oncology & Carcinogenesis
  • Nucleolin
  • Neoplasm Staging
 

Citation

APA
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ICMJE
MLA
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Peng, L., Liang, J., Wang, H., Song, X., Rashid, A., Gomez, H. F., … Evans, D. B. (2010). High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma. Clin Cancer Res, 16(14), 3734–3742. https://doi.org/10.1158/1078-0432.CCR-09-3411
Peng, Lan, John Liang, Hua Wang, Xianzhou Song, Asif Rashid, Henry F. Gomez, Lynda J. Corley, et al. “High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma.Clin Cancer Res 16, no. 14 (July 15, 2010): 3734–42. https://doi.org/10.1158/1078-0432.CCR-09-3411.
Peng L, Liang J, Wang H, Song X, Rashid A, Gomez HF, et al. High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma. Clin Cancer Res. 2010 Jul 15;16(14):3734–42.
Peng, Lan, et al. “High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma.Clin Cancer Res, vol. 16, no. 14, July 2010, pp. 3734–42. Pubmed, doi:10.1158/1078-0432.CCR-09-3411.
Peng L, Liang J, Wang H, Song X, Rashid A, Gomez HF, Corley LJ, Abbruzzese JL, Fleming JB, Evans DB. High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma. Clin Cancer Res. 2010 Jul 15;16(14):3734–3742.

Published In

Clin Cancer Res

DOI

EISSN

1557-3265

Publication Date

July 15, 2010

Volume

16

Issue

14

Start / End Page

3734 / 3742

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tissue Array Analysis
  • Retrospective Studies
  • RNA-Binding Proteins
  • Prognosis
  • Phosphoproteins
  • Pancreatic Neoplasms
  • Oncology & Carcinogenesis
  • Nucleolin
  • Neoplasm Staging