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Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells.

Publication ,  Journal Article
Frazier, ML; Fernández, E; de Llorens, R; Brown, NM; Pathak, S; Cleary, KR; Abbruzzese, JL; Berry, K; Olive, M; Le Maistre, A; Evans, DB
Published in: Int J Pancreatol
February 1996

CONCLUSION: We established a new human pancreatic adenocarcinoma cell line, MDAPanc-28. Studies on this new line indicate that it expressed both acinar and ductal gene products suggesting that the patterns of gene expression in the pancreatic adenocarcinoma from which this cell line arose have features similar to those of the protodifferentiated cells hypothesized by Rutter and his colleagues for the developing pancreas (1,2). BACKGROUND: The cell line arose from a tumor that, like most pancreatic adenocarcinomas, was ductal on the basis of its histological appearance. METHODS: Once the cell line was established in culture, they were subjected to cytogenetic analysis and tested for their ability to grow in nude mice. RNA from the cells was analyzed by Northern blot analysis and PCR of reverse transcribed cDNA for the expression of both acinar and duct cell gene products. DNA was analyzed for the presence of mutated K-ras at codon 12. RESULTS: The cell line expressed trypsin and ribonuclease RNA, which are considered to be acinar cell markers, and carbonic anhydrase II (CAII), which is considered to be a duct-cell markers. The histological appearance of xenografts in nude mice was similar to that of the tumor from which the cell line was established. The chromosome number varied between 46 and 60.

Duke Scholars

Published In

Int J Pancreatol

DOI

ISSN

0169-4197

Publication Date

February 1996

Volume

19

Issue

1

Start / End Page

31 / 38

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Trypsin
  • Transplantation, Heterologous
  • Ribonucleases
  • RNA, Neoplasm
  • Polymerase Chain Reaction
  • Point Mutation
  • Pancreatic Neoplasms
  • Molecular Sequence Data
  • Mice, Nude
 

Citation

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Frazier, M. L., Fernández, E., de Llorens, R., Brown, N. M., Pathak, S., Cleary, K. R., … Evans, D. B. (1996). Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells. Int J Pancreatol, 19(1), 31–38. https://doi.org/10.1007/BF02788373
Frazier, M. L., E. Fernández, R. de Llorens, N. M. Brown, S. Pathak, K. R. Cleary, J. L. Abbruzzese, et al. “Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells.Int J Pancreatol 19, no. 1 (February 1996): 31–38. https://doi.org/10.1007/BF02788373.
Frazier ML, Fernández E, de Llorens R, Brown NM, Pathak S, Cleary KR, et al. Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells. Int J Pancreatol. 1996 Feb;19(1):31–8.
Frazier, M. L., et al. “Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells.Int J Pancreatol, vol. 19, no. 1, Feb. 1996, pp. 31–38. Pubmed, doi:10.1007/BF02788373.
Frazier ML, Fernández E, de Llorens R, Brown NM, Pathak S, Cleary KR, Abbruzzese JL, Berry K, Olive M, Le Maistre A, Evans DB. Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and ductal cells. Int J Pancreatol. 1996 Feb;19(1):31–38.

Published In

Int J Pancreatol

DOI

ISSN

0169-4197

Publication Date

February 1996

Volume

19

Issue

1

Start / End Page

31 / 38

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Trypsin
  • Transplantation, Heterologous
  • Ribonucleases
  • RNA, Neoplasm
  • Polymerase Chain Reaction
  • Point Mutation
  • Pancreatic Neoplasms
  • Molecular Sequence Data
  • Mice, Nude