Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells.

Published

Journal Article

The influence of acidosis on the expression of the vascular endothelial growth factor (VEGF) gene was determined. FG human pancreatic adenocarcinoma cells were incubated for various time periods in media at a physiologically relevant pH level (6.7-7.4). The expression of VEGF mRNA and protein secretion was inversely correlated with pH in a pH- and time-dependent manner. Transient acidosis also activated the VEGF promoter/enhancer luciferase reporter, which was consistent with an increased VEGF gene transcription rate and VEGF mRNA half-life. These data indicated that acidosis transcriptionally and posttranscriptionally regulates VEGF expression, suggesting that an acidic tumor microenvironment contributes to tumor angiogenesis and progression.

Full Text

Duke Authors

Cited Authors

  • Shi, Q; Le, X; Wang, B; Abbruzzese, JL; Xiong, Q; He, Y; Xie, K

Published Date

  • June 2001

Published In

Volume / Issue

  • 20 / 28

Start / End Page

  • 3751 - 3756

PubMed ID

  • 11439338

Pubmed Central ID

  • 11439338

Electronic International Standard Serial Number (EISSN)

  • 1476-5594

International Standard Serial Number (ISSN)

  • 0950-9232

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1204500

Language

  • eng