NF-κB activity blockade impairs the angiogenic potential of human pancreatic cancer cells


Journal Article

The effect of blockade of NF-κB activity on human pancreatic cancer angiogenesis was determined in an orthotopic xenograft model. Highly metastatic L3.3 human pancreatic cancer cells, which expressed an elevated level of constitutive NF-κB activity, were transfected with a mutated IκBα (IκBαM). After implantation in the pancreas of nude mice, parental (L3.3) and control vector-transfected (L3.3-Neo) cells produced rapidly growing tumors and liver metastases, whereas IκBαM-transfected (L3.3-IκBαM) cells had decreased tumorigenicity and metastatic potential. NF-κB signaling blockade significantly inhibited the in vitro and in vivo expression of the major proangiogenic molecules vascular endothelial growth factor and interleukin-8 and decreased tumor vascular formation. These events were correlated with retarded tumor growth and suppression of metastasis. Collectively, these data suggest that suppression of tumorigenicity and metastasis by NF-κB blockade is due to impaired angiogenic potential of tumor cells. © 2003 Wiley-Liss, Inc.

Full Text

Duke Authors

Cited Authors

  • Xiong, HQ; Abbruzzese, JL; Lin, E; Wang, L; Zheng, L; Xie, K

Published Date

  • January 10, 2004

Published In

Volume / Issue

  • 108 / 2

Start / End Page

  • 181 - 188

International Standard Serial Number (ISSN)

  • 0020-7136

Digital Object Identifier (DOI)

  • 10.1002/ijc.11562

Citation Source

  • Scopus