Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF.

Journal Article (Journal Article)

We previously reported a protein expression profiling experiment conducted on human pancreatic tissues using 2D gel electrophoresis and mass spectrometry. Here, 18 spots that were identified in the gel at molecular weights more than 10 kDa lower than database values are characterized. The matrix-assisted laser desorption/ionization mass spectrometry coverage is sufficient to identify the protein region present in each spot. Most of the fragments correspond to processed chains and known structural or functional domains, which may result from limited proteolysis.

Full Text

Duke Authors

Cited Authors

  • Person, MD; Shen, J; Traner, A; Hensley, SC; Lo, H-H; Abbruzzese, JL; Li, D

Published Date

  • April 2006

Published In

Volume / Issue

  • 17 / 2

Start / End Page

  • 145 - 156

PubMed ID

  • 16741242

Pubmed Central ID

  • PMC2291776

International Standard Serial Number (ISSN)

  • 1524-0215

Language

  • eng

Conference Location

  • United States