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Molecular profiling of carcinoma of unknown primary and correlation with clinical evaluation.

Publication ,  Journal Article
Varadhachary, GR; Talantov, D; Raber, MN; Meng, C; Hess, KR; Jatkoe, T; Lenzi, R; Spigel, DR; Wang, Y; Greco, FA; Abbruzzese, JL; Hainsworth, JD
Published in: J Clin Oncol
September 20, 2008

PURPOSE: To evaluate the feasibility of a 10-gene reverse transcriptase polymerase chain reaction assay to identify the tissue of origin in patients with carcinoma of unknown primary (CUP) site. PATIENTS AND METHODS: Diagnostic biopsy formalin-fixed, paraffin-embedded (FFPE) specimens from 120 patients with CUP were collected retrospectively from Sarah Cannon Research Institute, Nashville, TN, and prospectively from The University of Texas M. D. Anderson Cancer Center, Houston, TX. Tissue of origin assignments by the assay were correlated with clinical and pathologic features and with response to therapy. RESULTS: The assay was successfully performed in 104 patients (87%), and a tissue of origin was assigned in 63 patients (61%). In the remaining 41 patients (39%), the molecular profiles were not specific for the six tumor types detectable by this assay. The tissues of origin most commonly identified were lung, pancreas, and colon; most of these patients had clinical and pathologic features consistent with these diagnoses. Patients with lung and pancreas profiles had poor response to treatment. Patients with colon cancer profiles had better response to colon cancer-specific therapies than they did to empiric CUP therapy with taxane/platinum regimens. Patients with ovarian cancer profiles were atypical, with widespread visceral metastases and a paucity of overt peritoneal involvement. CONCLUSION: This gene expression profiling assay was feasible using FFPE biopsy specimens and identified a putative tissue of origin in 61% of patients with CUP. In most patients, the assigned tissue of origin was compatible with clinicopathologic features and response to treatment. Prospective studies in which assay results are used to direct therapy are indicated.

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Published In

J Clin Oncol

DOI

EISSN

1527-7755

Publication Date

September 20, 2008

Volume

26

Issue

27

Start / End Page

4442 / 4448

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Retrospective Studies
  • Predictive Value of Tests
  • Oncology & Carcinogenesis
  • Neoplasms, Unknown Primary
  • Middle Aged
  • Male
  • Immunohistochemistry
  • Humans
  • Gene Expression Profiling
 

Citation

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Varadhachary, G. R., Talantov, D., Raber, M. N., Meng, C., Hess, K. R., Jatkoe, T., … Hainsworth, J. D. (2008). Molecular profiling of carcinoma of unknown primary and correlation with clinical evaluation. J Clin Oncol, 26(27), 4442–4448. https://doi.org/10.1200/JCO.2007.14.4378
Varadhachary, Gauri R., Dmitri Talantov, Martin N. Raber, Christina Meng, Kenneth R. Hess, Tim Jatkoe, Renato Lenzi, et al. “Molecular profiling of carcinoma of unknown primary and correlation with clinical evaluation.J Clin Oncol 26, no. 27 (September 20, 2008): 4442–48. https://doi.org/10.1200/JCO.2007.14.4378.
Varadhachary GR, Talantov D, Raber MN, Meng C, Hess KR, Jatkoe T, et al. Molecular profiling of carcinoma of unknown primary and correlation with clinical evaluation. J Clin Oncol. 2008 Sep 20;26(27):4442–8.
Varadhachary, Gauri R., et al. “Molecular profiling of carcinoma of unknown primary and correlation with clinical evaluation.J Clin Oncol, vol. 26, no. 27, Sept. 2008, pp. 4442–48. Pubmed, doi:10.1200/JCO.2007.14.4378.
Varadhachary GR, Talantov D, Raber MN, Meng C, Hess KR, Jatkoe T, Lenzi R, Spigel DR, Wang Y, Greco FA, Abbruzzese JL, Hainsworth JD. Molecular profiling of carcinoma of unknown primary and correlation with clinical evaluation. J Clin Oncol. 2008 Sep 20;26(27):4442–4448.

Published In

J Clin Oncol

DOI

EISSN

1527-7755

Publication Date

September 20, 2008

Volume

26

Issue

27

Start / End Page

4442 / 4448

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Retrospective Studies
  • Predictive Value of Tests
  • Oncology & Carcinogenesis
  • Neoplasms, Unknown Primary
  • Middle Aged
  • Male
  • Immunohistochemistry
  • Humans
  • Gene Expression Profiling