Methylomic markers of persistent childhood asthma: a longitudinal study of asthma-discordant monozygotic twins.

Published

Journal Article

Asthma is the most common chronic inflammatory disorder in children. The aetiology of asthma pathology is complex and highly heterogeneous, involving the interplay between genetic and environmental risk factors that is hypothesized to involve epigenetic processes. Our aim was to explore whether methylomic variation in early childhood is associated with discordance for asthma symptoms within monozygotic (MZ) twin pairs recruited from the Environmental Risk (E-Risk) longitudinal twin study. We also aimed to identify differences in DNA methylation that are associated with asthma that develops in childhood and persists into early adulthood as these may represent useful prognostic biomarkers.We examined genome-wide patterns of DNA methylation in buccal cell samples collected from 37 MZ twin pairs discordant for asthma at age 10. DNA methylation at individual CpG sites demonstrated significant variability within discordant MZ twin pairs with the top-ranked nominally significant differentially methylated position (DMP) located in the HGSNAT gene. We stratified our analysis by assessing DNA methylation differences in a sub-group of MZ twin pairs who remained persistently discordant for asthma at age 18. The top-ranked nominally significant DMP associated with persisting asthma is located in the vicinity of the HLX gene, which has been previously implicated in childhood asthma.We identified DNA methylation differences associated with childhood asthma in peripheral DNA samples from discordant MZ twin pairs. Our data suggest that differences in DNA methylation associated with childhood asthma which persists into early adulthood are distinct from those associated with asthma which remits.

Full Text

Duke Authors

Cited Authors

  • Murphy, TM; Wong, CCY; Arseneault, L; Burrage, J; Macdonald, R; Hannon, E; Fisher, HL; Ambler, A; Moffitt, TE; Caspi, A; Mill, J

Published Date

  • January 2015

Published In

Volume / Issue

  • 7 /

Start / End Page

  • 130 -

PubMed ID

  • 26691723

Pubmed Central ID

  • 26691723

Electronic International Standard Serial Number (EISSN)

  • 1868-7083

International Standard Serial Number (ISSN)

  • 1868-7075

Digital Object Identifier (DOI)

  • 10.1186/s13148-015-0163-4

Language

  • eng