Prevalence of AAV1 neutralizing antibodies and consequences for a clinical trial of gene transfer for advanced heart failure.

Published

Journal Article

Adeno-associated virus serotype 1 (AAV1) has many advantages as a gene therapy vector, but the presence of pre-existing neutralizing antibodies (NAbs) is an important limitation. This study was designed to determine: (1) characteristics of AAV NAbs in human subjects, (2) prevalence of AAV1 NAbs in heart failure patients and (3) utility of aggressive immunosuppressive therapy in reducing NAb seroconversion in an animal model. NAb titers were assessed in a cohort of heart failure patients and in patients screened for a clinical trial of gene therapy with AAV1 carrying the sarcoplasmic reticulum calcium ATPase gene (AAV1/SERCA2a). AAV1 NAbs were found in 59.5% of 1552 heart failure patients. NAb prevalence increased with age (P=0.001) and varied geographically. The pattern of NAb titers suggested that exposure is against AAV2, with AAV1 NAb seropositivity due to crossreactivity. The effects of immunosuppression on NAb formation were tested in mini-pigs treated with immunosuppressant therapy before, during and after a single AAV1/SERCA2a infusion. Aggressive immunosuppression did not prevent formation of AAV1 NAbs. We conclude that immunosuppression is unlikely to be a viable solution for repeat AAV1 dosing. Strategies to reduce NAbs in heart failure patients are needed to increase eligibility for gene transfer using AAV vectors.

Full Text

Duke Authors

Cited Authors

  • Greenberg, B; Butler, J; Felker, GM; Ponikowski, P; Voors, AA; Pogoda, JM; Provost, R; Guerrero, J; Hajjar, RJ; Zsebo, KM

Published Date

  • March 2016

Published In

Volume / Issue

  • 23 / 3

Start / End Page

  • 313 - 319

PubMed ID

  • 26699914

Pubmed Central ID

  • 26699914

Electronic International Standard Serial Number (EISSN)

  • 1476-5462

Digital Object Identifier (DOI)

  • 10.1038/gt.2015.109

Language

  • eng

Conference Location

  • England