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An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina.

Publication ,  Journal Article
Visser, JJ; Cheng, Y; Perry, SC; Chastain, AB; Parsa, B; Masri, SS; Ray, TA; Kay, JN; Wojtowicz, WM
Published in: Elife
December 2, 2015

In the inner plexiform layer (IPL) of the mouse retina, ~70 neuronal subtypes organize their neurites into an intricate laminar structure that underlies visual processing. To find recognition proteins involved in lamination, we utilized microarray data from 13 subtypes to identify differentially-expressed extracellular proteins and performed a high-throughput biochemical screen. We identified ~50 previously-unknown receptor-ligand pairs, including new interactions among members of the FLRT and Unc5 families. These proteins show laminar-restricted IPL localization and induce attraction and/or repulsion of retinal neurites in culture, placing them in an ideal position to mediate laminar targeting. Consistent with a repulsive role in arbor lamination, we observed complementary expression patterns for one interaction pair, FLRT2-Unc5C, in vivo. Starburst amacrine cells and their synaptic partners, ON-OFF direction-selective ganglion cells, express FLRT2 and are repelled by Unc5C. These data suggest a single molecular mechanism may have been co-opted by synaptic partners to ensure joint laminar restriction.

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

December 2, 2015

Volume

4

Start / End Page

e08149

Location

England

Related Subject Headings

  • Retina
  • Receptors, Cell Surface
  • Proteome
  • Protein Interaction Mapping
  • Protein Binding
  • Neurons
  • Netrin Receptors
  • Microarray Analysis
  • Mice
  • Membrane Glycoproteins
 

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Visser, J. J., Cheng, Y., Perry, S. C., Chastain, A. B., Parsa, B., Masri, S. S., … Wojtowicz, W. M. (2015). An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. Elife, 4, e08149. https://doi.org/10.7554/eLife.08149
Visser, Jasper J., Yolanda Cheng, Steven C. Perry, Andrew Benjamin Chastain, Bayan Parsa, Shatha S. Masri, Thomas A. Ray, Jeremy N. Kay, and Woj M. Wojtowicz. “An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina.Elife 4 (December 2, 2015): e08149. https://doi.org/10.7554/eLife.08149.
Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, et al. An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. Elife. 2015 Dec 2;4:e08149.
Visser, Jasper J., et al. “An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina.Elife, vol. 4, Dec. 2015, p. e08149. Pubmed, doi:10.7554/eLife.08149.
Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM. An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. Elife. 2015 Dec 2;4:e08149.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

December 2, 2015

Volume

4

Start / End Page

e08149

Location

England

Related Subject Headings

  • Retina
  • Receptors, Cell Surface
  • Proteome
  • Protein Interaction Mapping
  • Protein Binding
  • Neurons
  • Netrin Receptors
  • Microarray Analysis
  • Mice
  • Membrane Glycoproteins