Biomimetic Surface Patterning Promotes Mesenchymal Stem Cell Differentiation.
Published
Journal Article
Both chemical and mechanical stimuli can dramatically influence cell behavior. By optimizing the signals cells experience, it may be possible to control the behavior of therapeutic cell populations. In this work, biomimetic geometries of adhesive ligands, which recapitulate the morphology of mature cells, are used to direct human mesenchymal stem cell (HMSC) differentiation toward a desired lineage. Specifically, adipocytes cultured in 2D are imaged and used to develop biomimetic virtual masks used in laser scanning lithography to form patterned fibronectin surfaces. The impact of adipocyte-derived pattern geometry on HMSC differentiation is compared to the behavior of HMSCs cultured on square and circle geometries, as well as adipocyte-derived patterns modified to include high stress regions. HMSCs on adipocyte mimetic geometries demonstrate greater adipogenesis than HMSCs on the other patterns. Greater than 45% of all HMSCs cultured on adipocyte mimetic patterns underwent adipogenesis as compared to approximately 19% of cells on modified adipocyte patterns with higher stress regions. These results are attributed to variations in cytoskeletal tension experienced by cells on the different protein micropatterns. The effects of geometry on adipogenesis are mitigated by the incorporation of a cytoskeletal protein inhibitor; exposure to this inhibitor leads to increased adipogenesis on all patterns examined.
Full Text
Duke Authors
Cited Authors
- Shukla, A; Slater, JH; Culver, JC; Dickinson, ME; West, JL
Published Date
- August 2016
Published In
Volume / Issue
- 8 / 34
Start / End Page
- 21883 - 21892
PubMed ID
- 26674708
Pubmed Central ID
- 26674708
Electronic International Standard Serial Number (EISSN)
- 1944-8252
International Standard Serial Number (ISSN)
- 1944-8244
Digital Object Identifier (DOI)
- 10.1021/acsami.5b08978
Language
- eng