Rapid Exchange Between Free and Bound States in RNA-Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release.
Published
Journal Article
A combination of solution NMR, dynamic light scattering (DLS), and fluorescence quenching assays were employed to obtain insights into the dynamics and structural features of a polyplex system consisting of HIV-1 transactivation response element (TAR) and PEGylated generation 5 poly(amidoamine) dendrimer (G5-PEG). NMR chemical shift mapping and (13)C spin relaxation based dynamics measurements depict the polyplex system as a highly dynamic assembly where the RNA, with its local structure and dynamics preserved, rapidly exchanges (
Full Text
Duke Authors
Cited Authors
- Shakya, A; Dougherty, CA; Xue, Y; Al-Hashimi, HM; Banaszak Holl, MM
Published Date
- January 11, 2016
Published In
Volume / Issue
- 17 / 1
Start / End Page
- 154 - 164
PubMed ID
- 26595195
Pubmed Central ID
- 26595195
Electronic International Standard Serial Number (EISSN)
- 1526-4602
Digital Object Identifier (DOI)
- 10.1021/acs.biomac.5b01280
Language
- eng
Conference Location
- United States