Rapid Exchange Between Free and Bound States in RNA-Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release.

Journal Article (Journal Article)

A combination of solution NMR, dynamic light scattering (DLS), and fluorescence quenching assays were employed to obtain insights into the dynamics and structural features of a polyplex system consisting of HIV-1 transactivation response element (TAR) and PEGylated generation 5 poly(amidoamine) dendrimer (G5-PEG). NMR chemical shift mapping and (13)C spin relaxation based dynamics measurements depict the polyplex system as a highly dynamic assembly where the RNA, with its local structure and dynamics preserved, rapidly exchanges (

Full Text

Duke Authors

Cited Authors

  • Shakya, A; Dougherty, CA; Xue, Y; Al-Hashimi, HM; Banaszak Holl, MM

Published Date

  • January 11, 2016

Published In

Volume / Issue

  • 17 / 1

Start / End Page

  • 154 - 164

PubMed ID

  • 26595195

Pubmed Central ID

  • PMC5070374

Electronic International Standard Serial Number (EISSN)

  • 1526-4602

Digital Object Identifier (DOI)

  • 10.1021/acs.biomac.5b01280

Language

  • eng

Conference Location

  • United States