A physical map of the human Y chromosome.

Published

Journal Article

The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.

Full Text

Duke Authors

Cited Authors

  • Tilford, CA; Kuroda-Kawaguchi, T; Skaletsky, H; Rozen, S; Brown, LG; Rosenberg, M; McPherson, JD; Wylie, K; Sekhon, M; Kucaba, TA; Waterston, RH; Page, DC

Published Date

  • February 15, 2001

Published In

Volume / Issue

  • 409 / 6822

Start / End Page

  • 943 - 945

PubMed ID

  • 11237016

Pubmed Central ID

  • 11237016

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/35057170

Language

  • eng

Conference Location

  • England