A physical map of the human Y chromosome.
Published
Journal Article
The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.
Full Text
Duke Authors
Cited Authors
- Tilford, CA; Kuroda-Kawaguchi, T; Skaletsky, H; Rozen, S; Brown, LG; Rosenberg, M; McPherson, JD; Wylie, K; Sekhon, M; Kucaba, TA; Waterston, RH; Page, DC
Published Date
- February 15, 2001
Published In
Volume / Issue
- 409 / 6822
Start / End Page
- 943 - 945
PubMed ID
- 11237016
Pubmed Central ID
- 11237016
International Standard Serial Number (ISSN)
- 0028-0836
Digital Object Identifier (DOI)
- 10.1038/35057170
Language
- eng
Conference Location
- England