Tuberculosis genes expressed during persistence and reactivation in the resistant rabbit model.

Published

Journal Article

As previously published, after aerosol infection with Mycobacterium tuberculosis H37Rv, New Zealand white rabbits established infection with active bacillary replication, but later contained disease to a paucibacillary state through an effective adaptive response consistent with latency. Despite the heterogeneity among outbred rabbits, the resistant response was uniform. Immunosuppression resulted in reactivation with increased lung bacillary burden. Using this rabbit model, we isolated bacillary RNA from infected rabbit lungs and assessed transcriptional profiles of bacillary genes using RT-PCR to examine genes differentially regulated during active replication, persistence, steroid-induced reactivation, and post-steroid immune reconstitution. Genes involved in hypoxia response (fdxA), resuscitation promoting factors (rpfB), and DNA repair pathways (Rv2191) may be important in bacillary persistence. Further investigation into these gene pathways is warranted.

Full Text

Duke Authors

Cited Authors

  • Kesavan, AK; Brooks, M; Tufariello, J; Chan, J; Manabe, YC

Published Date

  • January 2009

Published In

Volume / Issue

  • 89 / 1

Start / End Page

  • 17 - 21

PubMed ID

  • 18948063

Pubmed Central ID

  • 18948063

Electronic International Standard Serial Number (EISSN)

  • 1873-281X

Digital Object Identifier (DOI)

  • 10.1016/j.tube.2008.08.004

Language

  • eng

Conference Location

  • Scotland