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Knock-down of LAR protein tyrosine phosphatase induces insulin resistance.

Publication ,  Journal Article
Mander, A; Hodgkinson, CP; Sale, GJ
Published in: FEBS Lett
June 6, 2005

To test the role of the leukocyte common antigen-related protein tyrosine phosphatase (LAR) as a regulator of insulin receptor (IR) signalling, an siRNA probe against LAR was developed. Knock-down of LAR induced post-receptor insulin resistance with the insulin-induced activation of PKB/Akt and MAP kinases markedly inhibited. The phosphorylation and dephosphorylation of the IR and insulin receptor substrate (IRS) proteins were unaffected by LAR knock-down. These results identify LAR as a crucial regulator of the sensitivity of two key insulin signalling pathways to insulin. Moreover, the siRNA probe provides a molecular tool of general applicability for further dissecting the precise targets and roles of LAR.

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Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

June 6, 2005

Volume

579

Issue

14

Start / End Page

3024 / 3028

Location

England

Related Subject Headings

  • Substrate Specificity
  • Signal Transduction
  • Receptors, Cell Surface
  • Receptor-Like Protein Tyrosine Phosphatases, Class 4
  • Receptor, Insulin
  • RNA, Small Interfering
  • Protein Tyrosine Phosphatases
  • Phosphotyrosine
  • Insulin Resistance
  • Insulin
 

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Mander, A., Hodgkinson, C. P., & Sale, G. J. (2005). Knock-down of LAR protein tyrosine phosphatase induces insulin resistance. FEBS Lett, 579(14), 3024–3028. https://doi.org/10.1016/j.febslet.2005.04.057
Mander, Ann, Conrad P. Hodgkinson, and Graham J. Sale. “Knock-down of LAR protein tyrosine phosphatase induces insulin resistance.FEBS Lett 579, no. 14 (June 6, 2005): 3024–28. https://doi.org/10.1016/j.febslet.2005.04.057.
Mander A, Hodgkinson CP, Sale GJ. Knock-down of LAR protein tyrosine phosphatase induces insulin resistance. FEBS Lett. 2005 Jun 6;579(14):3024–8.
Mander, Ann, et al. “Knock-down of LAR protein tyrosine phosphatase induces insulin resistance.FEBS Lett, vol. 579, no. 14, June 2005, pp. 3024–28. Pubmed, doi:10.1016/j.febslet.2005.04.057.
Mander A, Hodgkinson CP, Sale GJ. Knock-down of LAR protein tyrosine phosphatase induces insulin resistance. FEBS Lett. 2005 Jun 6;579(14):3024–3028.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

June 6, 2005

Volume

579

Issue

14

Start / End Page

3024 / 3028

Location

England

Related Subject Headings

  • Substrate Specificity
  • Signal Transduction
  • Receptors, Cell Surface
  • Receptor-Like Protein Tyrosine Phosphatases, Class 4
  • Receptor, Insulin
  • RNA, Small Interfering
  • Protein Tyrosine Phosphatases
  • Phosphotyrosine
  • Insulin Resistance
  • Insulin