Knock-down of LAR protein tyrosine phosphatase induces insulin resistance.

Journal Article (Journal Article)

To test the role of the leukocyte common antigen-related protein tyrosine phosphatase (LAR) as a regulator of insulin receptor (IR) signalling, an siRNA probe against LAR was developed. Knock-down of LAR induced post-receptor insulin resistance with the insulin-induced activation of PKB/Akt and MAP kinases markedly inhibited. The phosphorylation and dephosphorylation of the IR and insulin receptor substrate (IRS) proteins were unaffected by LAR knock-down. These results identify LAR as a crucial regulator of the sensitivity of two key insulin signalling pathways to insulin. Moreover, the siRNA probe provides a molecular tool of general applicability for further dissecting the precise targets and roles of LAR.

Full Text

Duke Authors

Cited Authors

  • Mander, A; Hodgkinson, CP; Sale, GJ

Published Date

  • June 6, 2005

Published In

Volume / Issue

  • 579 / 14

Start / End Page

  • 3024 - 3028

PubMed ID

  • 15896785

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/j.febslet.2005.04.057


  • eng

Conference Location

  • England