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Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking.

Publication ,  Journal Article
Hodgkinson, CP; Mander, A; Sale, GJ
Published in: Diabetologia
August 2005

AIMS/HYPOTHESIS: Insulin-stimulated glucose transport requires a signalling cascade through kinases protein kinase (PK) Czeta/lambda and PKB that leads to movement of GLUT4 vesicles to the plasma membrane. The aim of this study was to identify missing links between the upstream insulin-regulated kinases and the GLUT4 vesicle trafficking system. MATERIALS AND METHODS: A yeast two-hybrid screen was conducted, using as bait full-length mouse munc18c, a protein known to be part of the GLUT4 vesicle trafficking machinery. RESULTS: The yeast two-hybrid screen identified PKCzeta as a novel interactor with munc18c. Glutathione S transferase (GST) pull-downs with GST-tagged munc18c constructs confirmed the interaction, mapped a key region of munc18c that binds PKCzeta to residues 295-338 and showed that the N-terminal region of PKCzeta was required for the interaction. Endogenous munc18c was shown to associate with endogenous PKCzeta in vivo in various cell types. Importantly, insulin stimulation increased the association by approximately three-fold. Moreover, disruption of PKCzeta binding to munc18c by deletion of residues 295-338 of munc18c or deletion of the N-terminal region of PKCzeta markedly inhibited the ability of insulin to stimulate glucose uptake or GLUT4 translocation. CONCLUSIONS/INTERPRETATION: We have identified a physiological interaction between munc18c and PKCzeta that is insulin-regulated. This establishes a link between a kinase (PKCzeta) involved in the insulin signalling cascade and a known component of the GLUT4 vesicle trafficking pathway (munc18c). The results indicate that PKCzeta regulates munc18c and suggest a model whereby insulin triggers the docking of PKCzeta to munc18c, resulting in enhanced GLUT4 translocation to the plasma membrane.

Duke Scholars

Published In

Diabetologia

DOI

ISSN

0012-186X

Publication Date

August 2005

Volume

48

Issue

8

Start / End Page

1627 / 1636

Location

Germany

Related Subject Headings

  • Vesicular Transport Proteins
  • Transfection
  • Protein Kinase C
  • Plasmids
  • Phosphorylation
  • Nerve Tissue Proteins
  • Muscle Proteins
  • Munc18 Proteins
  • Monosaccharide Transport Proteins
  • Insulin
 

Citation

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MLA
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Hodgkinson, C. P., Mander, A., & Sale, G. J. (2005). Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking. Diabetologia, 48(8), 1627–1636. https://doi.org/10.1007/s00125-005-1819-y
Hodgkinson, C. P., A. Mander, and G. J. Sale. “Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking.Diabetologia 48, no. 8 (August 2005): 1627–36. https://doi.org/10.1007/s00125-005-1819-y.
Hodgkinson CP, Mander A, Sale GJ. Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking. Diabetologia. 2005 Aug;48(8):1627–36.
Hodgkinson, C. P., et al. “Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking.Diabetologia, vol. 48, no. 8, Aug. 2005, pp. 1627–36. Pubmed, doi:10.1007/s00125-005-1819-y.
Hodgkinson CP, Mander A, Sale GJ. Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking. Diabetologia. 2005 Aug;48(8):1627–1636.
Journal cover image

Published In

Diabetologia

DOI

ISSN

0012-186X

Publication Date

August 2005

Volume

48

Issue

8

Start / End Page

1627 / 1636

Location

Germany

Related Subject Headings

  • Vesicular Transport Proteins
  • Transfection
  • Protein Kinase C
  • Plasmids
  • Phosphorylation
  • Nerve Tissue Proteins
  • Muscle Proteins
  • Munc18 Proteins
  • Monosaccharide Transport Proteins
  • Insulin