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Cocaine downregulates beta-adrenergic receptors in pregnant sheep myometrium.

Publication ,  Journal Article
Wang, FL; Gauvin, JM; Dombrowski, MP; Smith, YR; Christopher, KA; Hurd, WW
Published in: Reprod Toxicol
1996

Cocaine abuse is associated with premature labor. Although cocaine is known to competitively inhibit beta-adrenergic receptor binding, cocaine's effect on receptor downregulation is uncertain. This study was designed to determine the in vitro effect of cocaine on downregulation of beta-adrenergic receptors in pregnant myometrium. Pregnant sheep myometrium was incubated with either cocaine, isoproterenol, or a cocaine metabolite, benzoylecgonine. Membrane fractions were assayed for beta-adrenergic receptors using (125I)-cyanopindolol and the beta 2-adrenergic antagonist ICI 118,551. We found that cocaine (10(-6) to 10(-4) mol/L), but not benzoylecgonine, downregulated both beta 1- and beta 2-adrenergic receptors, but did not further augment receptor downregulation by isoproterenol. The 46% decrease in beta-adrenergic receptors seen after exposure to cocaine was similar to the 53% decrease seen after isoproterenol. We hypothesize downregulation of beta-adrenergic receptors by cocaine may play a role in the association of cocaine abuse with premature labor.

Duke Scholars

Published In

Reprod Toxicol

DOI

ISSN

0890-6238

Publication Date

1996

Volume

10

Issue

2

Start / End Page

119 / 123

Location

United States

Related Subject Headings

  • Toxicology
  • Sheep
  • Receptors, Adrenergic, beta
  • Pregnancy
  • Myometrium
  • Isoproterenol
  • Female
  • Down-Regulation
  • Cocaine
  • Animals
 

Citation

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ICMJE
MLA
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Wang, F. L., Gauvin, J. M., Dombrowski, M. P., Smith, Y. R., Christopher, K. A., & Hurd, W. W. (1996). Cocaine downregulates beta-adrenergic receptors in pregnant sheep myometrium. Reprod Toxicol, 10(2), 119–123. https://doi.org/10.1016/0890-6238(95)02053-5
Wang, F. L., J. M. Gauvin, M. P. Dombrowski, Y. R. Smith, K. A. Christopher, and W. W. Hurd. “Cocaine downregulates beta-adrenergic receptors in pregnant sheep myometrium.Reprod Toxicol 10, no. 2 (1996): 119–23. https://doi.org/10.1016/0890-6238(95)02053-5.
Wang FL, Gauvin JM, Dombrowski MP, Smith YR, Christopher KA, Hurd WW. Cocaine downregulates beta-adrenergic receptors in pregnant sheep myometrium. Reprod Toxicol. 1996;10(2):119–23.
Wang, F. L., et al. “Cocaine downregulates beta-adrenergic receptors in pregnant sheep myometrium.Reprod Toxicol, vol. 10, no. 2, 1996, pp. 119–23. Pubmed, doi:10.1016/0890-6238(95)02053-5.
Wang FL, Gauvin JM, Dombrowski MP, Smith YR, Christopher KA, Hurd WW. Cocaine downregulates beta-adrenergic receptors in pregnant sheep myometrium. Reprod Toxicol. 1996;10(2):119–123.
Journal cover image

Published In

Reprod Toxicol

DOI

ISSN

0890-6238

Publication Date

1996

Volume

10

Issue

2

Start / End Page

119 / 123

Location

United States

Related Subject Headings

  • Toxicology
  • Sheep
  • Receptors, Adrenergic, beta
  • Pregnancy
  • Myometrium
  • Isoproterenol
  • Female
  • Down-Regulation
  • Cocaine
  • Animals