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Overexpression of hMTH in peripheral lymphocytes and risk of prostate cancer: a case-control analysis.

Publication ,  Journal Article
Liu, Z; Wang, L-E; Strom, SS; Spitz, MR; Babaian, RJ; DiGiovanni, J; Wei, Q
Published in: Mol Carcinog
March 2003

Oxidative damage is an important factor in prostate carcinogenesis, and overexpression of human MutT homolog (hMTH), a repair gene that removes oxidative damage, is a molecular marker of cellular oxidative stress. Therefore, we tested the hypothesis that overexpression of hMTH in unaffected (normal) surrogate tissue is associated with risk of prostate cancer in a pilot study of 51 patients with diagnosed prostate cancer and 50 age- and ethnicity-matched controls. Total RNA was extracted from phytohemagglutinin-stimulated peripheral blood lymphocytes of these subjects. We performed the real-time reverse transcription-polymerase chain reaction assay to evaluate the relative mRNA expression of three oxidative-damage-repair genes, human MutM homolog (hMMH), hMTH, and human MutY homolog (hMYH), with beta-actin and human O(6)-methylguanine DNA methyltransferase (hMGMT) as the internal controls. The relative gene expression levels of hMMH and hMTH were borderline higher in the cases than in controls (15.3% and 28.8% higher, respectively; P = 0.046 and P = 0.035, respectively), whereas no increase was observed for hMYH and hMGMT. With the median of the controls' values as the cutoff point, we observed that a high expression level of hMTH, but not of other genes, was associated with a significantly increased risk of prostate cancer (odds ratio = 2.62; 95% confidence interval = 1.13-6.75) after adjustment for age and ethnicity. These results suggested that increased expression of hMTH in peripheral lymphocytes may be a risk factor for prostate cancer and support our priori hypothesis. Although our findings were biologically plausible and consistent with the literature, they were preliminary and need to be confirmed in larger studies. In addition, a correlation between the expression level of hMTH and the level of oxidative DNA damage in the target tissues needs to be established as well.

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Published In

Mol Carcinog

DOI

ISSN

0899-1987

Publication Date

March 2003

Volume

36

Issue

3

Start / End Page

123 / 129

Location

United States

Related Subject Headings

  • White People
  • Texas
  • Risk Factors
  • Regression Analysis
  • Reference Values
  • RNA, Messenger
  • Prostatic Neoplasms
  • Pilot Projects
  • Phytohemagglutinins
  • Phosphoric Monoester Hydrolases
 

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Liu, Z., Wang, L.-E., Strom, S. S., Spitz, M. R., Babaian, R. J., DiGiovanni, J., & Wei, Q. (2003). Overexpression of hMTH in peripheral lymphocytes and risk of prostate cancer: a case-control analysis. Mol Carcinog, 36(3), 123–129. https://doi.org/10.1002/mc.10108
Liu, Zhensheng, Li-E Wang, Sara S. Strom, Margaret R. Spitz, Richard J. Babaian, John DiGiovanni, and Qingyi Wei. “Overexpression of hMTH in peripheral lymphocytes and risk of prostate cancer: a case-control analysis.Mol Carcinog 36, no. 3 (March 2003): 123–29. https://doi.org/10.1002/mc.10108.
Liu Z, Wang L-E, Strom SS, Spitz MR, Babaian RJ, DiGiovanni J, et al. Overexpression of hMTH in peripheral lymphocytes and risk of prostate cancer: a case-control analysis. Mol Carcinog. 2003 Mar;36(3):123–9.
Liu, Zhensheng, et al. “Overexpression of hMTH in peripheral lymphocytes and risk of prostate cancer: a case-control analysis.Mol Carcinog, vol. 36, no. 3, Mar. 2003, pp. 123–29. Pubmed, doi:10.1002/mc.10108.
Liu Z, Wang L-E, Strom SS, Spitz MR, Babaian RJ, DiGiovanni J, Wei Q. Overexpression of hMTH in peripheral lymphocytes and risk of prostate cancer: a case-control analysis. Mol Carcinog. 2003 Mar;36(3):123–129.
Journal cover image

Published In

Mol Carcinog

DOI

ISSN

0899-1987

Publication Date

March 2003

Volume

36

Issue

3

Start / End Page

123 / 129

Location

United States

Related Subject Headings

  • White People
  • Texas
  • Risk Factors
  • Regression Analysis
  • Reference Values
  • RNA, Messenger
  • Prostatic Neoplasms
  • Pilot Projects
  • Phytohemagglutinins
  • Phosphoric Monoester Hydrolases