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Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line.

Publication ,  Journal Article
Widra, EA; Weeraratna, A; Stepp, MA; Stillman, RJ; Patierno, SR
Published in: Mol Hum Reprod
July 1997

In order to test the hypothesis that integrin and uteroglobin (UG) expression in cultured endometrial cells are affected by hormone treatment, Ishikawa-CH endometrial cancer cells were cultured and exposed to oestradiol or oestradiol and progesterone regimens and assayed using immunohistochemistry. We evaluated the intensity of immunohistochemical staining for the integrin monomers alpha(v) and beta1, the dimers alpha(v)beta3 and alpha(v)beta6, and for the secretory protein uteroglobin under various experimental conditions. Cells grown in control media stained positively for the integrin monomers alpha(v) and beta1, the dimer alpha(v)beta3, and for UG. Oestradiol and sequential oestradiol/progesterone reversibly suppressed staining for the dimer alpha(v)beta3. Hormone treatment had no effect on the staining of the beta1 and alpha(v) monomers or UG. The alpha(v)beta6 dimer antibody did not stain under any experimental treatment conditions. These data indicate that expression of the integrin complex alpha(v)beta3 is reversibly suppressed by oestradiol in Ishikawa cells and that these cells may be a good model for studying hormone-driven molecular changes in endometrium.

Duke Scholars

Published In

Mol Hum Reprod

DOI

ISSN

1360-9947

Publication Date

July 1997

Volume

3

Issue

7

Start / End Page

563 / 568

Location

England

Related Subject Headings

  • Uteroglobin
  • Tumor Cells, Cultured
  • Progesterone
  • Obstetrics & Reproductive Medicine
  • Integrins
  • Humans
  • Female
  • Estradiol
  • Endometrium
  • Endometrial Neoplasms
 

Citation

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Widra, E. A., Weeraratna, A., Stepp, M. A., Stillman, R. J., & Patierno, S. R. (1997). Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line. Mol Hum Reprod, 3(7), 563–568. https://doi.org/10.1093/molehr/3.7.563
Widra, E. A., A. Weeraratna, M. A. Stepp, R. J. Stillman, and S. R. Patierno. “Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line.Mol Hum Reprod 3, no. 7 (July 1997): 563–68. https://doi.org/10.1093/molehr/3.7.563.
Widra EA, Weeraratna A, Stepp MA, Stillman RJ, Patierno SR. Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line. Mol Hum Reprod. 1997 Jul;3(7):563–8.
Widra, E. A., et al. “Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line.Mol Hum Reprod, vol. 3, no. 7, July 1997, pp. 563–68. Pubmed, doi:10.1093/molehr/3.7.563.
Widra EA, Weeraratna A, Stepp MA, Stillman RJ, Patierno SR. Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line. Mol Hum Reprod. 1997 Jul;3(7):563–568.
Journal cover image

Published In

Mol Hum Reprod

DOI

ISSN

1360-9947

Publication Date

July 1997

Volume

3

Issue

7

Start / End Page

563 / 568

Location

England

Related Subject Headings

  • Uteroglobin
  • Tumor Cells, Cultured
  • Progesterone
  • Obstetrics & Reproductive Medicine
  • Integrins
  • Humans
  • Female
  • Estradiol
  • Endometrium
  • Endometrial Neoplasms