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Ca(2+)-regulated serine protease associated with the nuclear scaffold.

Publication ,  Journal Article
Clawson, GA; Norbeck, LL; Hatem, CL; Rhodes, C; Amiri, P; McKerrow, JH; Patierno, SR; Fiskum, G
Published in: Cell Growth Differ
November 1992

The nuclear scaffold (NS) is a proteinaceous network of orthogonally arrayed intermediate filament proteins, termed lamins, which is responsible for nuclear structure. Recent work has demonstrated that a subset of lamins A/C is proteolytically cleaved to produce an ATP-binding protein. This proteolytic cleavage is accomplished by a NS protease activity, which shows a considerable selectivity for lamins A/C and is stringently regulated by Ca2+ in vitro, suggesting that it might also participate in control of NS breakdown in various scenarios. Here, we identify the major NS protease as a novel serine protease with a predominantly chymotryptic-like substrate preference, and we show that even transient perturbations in cytosolic Ca2+ have significant effects on the NS protease activity. This NS protease activity shows extensive similarities to the multicatalytic proteinase complex. In addition to a potential role in control of NS breakdown at mitosis and/or under pathological conditions, this NS protease is also strategically located for other functions, such as inactivation of various oncogenic proteins or maturation-promoting factor.

Duke Scholars

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

November 1992

Volume

3

Issue

11

Start / End Page

827 / 838

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Substrate Specificity
  • Species Specificity
  • Serine Endopeptidases
  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Rats, Sprague-Dawley
  • Rats
  • Peptides
  • Oncology & Carcinogenesis
 

Citation

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Clawson, G. A., Norbeck, L. L., Hatem, C. L., Rhodes, C., Amiri, P., McKerrow, J. H., … Fiskum, G. (1992). Ca(2+)-regulated serine protease associated with the nuclear scaffold. Cell Growth Differ, 3(11), 827–838.
Clawson, G. A., L. L. Norbeck, C. L. Hatem, C. Rhodes, P. Amiri, J. H. McKerrow, S. R. Patierno, and G. Fiskum. “Ca(2+)-regulated serine protease associated with the nuclear scaffold.Cell Growth Differ 3, no. 11 (November 1992): 827–38.
Clawson GA, Norbeck LL, Hatem CL, Rhodes C, Amiri P, McKerrow JH, et al. Ca(2+)-regulated serine protease associated with the nuclear scaffold. Cell Growth Differ. 1992 Nov;3(11):827–38.
Clawson, G. A., et al. “Ca(2+)-regulated serine protease associated with the nuclear scaffold.Cell Growth Differ, vol. 3, no. 11, Nov. 1992, pp. 827–38.
Clawson GA, Norbeck LL, Hatem CL, Rhodes C, Amiri P, McKerrow JH, Patierno SR, Fiskum G. Ca(2+)-regulated serine protease associated with the nuclear scaffold. Cell Growth Differ. 1992 Nov;3(11):827–838.

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

November 1992

Volume

3

Issue

11

Start / End Page

827 / 838

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Substrate Specificity
  • Species Specificity
  • Serine Endopeptidases
  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Rats, Sprague-Dawley
  • Rats
  • Peptides
  • Oncology & Carcinogenesis