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Clastogenicity of lead chromate particles in hamster and human cells.

Publication ,  Journal Article
Wise, JP; Leonard, JC; Patierno, SR
Published in: Mutat Res
January 1992

Several insoluble compounds of chromium, such as lead chromate, are respiratory carcinogens in experimental animals and suspected to be so in humans. Lead chromate induces neoplastic transformation in cultured cells but the mechanism of genotoxicity is unknown. We examined the effect of lead chromate on the integrity of chromosomes of Chinese hamster ovary (CHO) and human foreskin fibroblasts (HFF) after a 24-h exposure. At 0.4 microgram/cm2, 0.8 microgram/cm2, 2 microgram/cm2 and 8 microgram/cm2 lead chromate particles reduced survival of CHO cells to 86%, 62%, 2% and less than 1% respectively. These concentrations induced a dose-dependent 4-19-fold increase in the percent metaphases with damage. The HFF cells exhibited higher sensitivity in both cytotoxicity and clastogenicity. The spectrum of damage observed for both cell types was primarily achromatic lesions affecting one or both chromatids. To test for particle dissolution effects, CHO cells were treated for 24 h with either clarified medium that had been incubated for 24 h with lead chromate particles, or clarified medium that had been pre-conditioned by CHO cells treated with lead chromate particles for 24 h. No damage was detected in these cells, indicating that extracellular dissolution into ionic lead and chromate did not contribute to the genotoxicity. This is consistent with a previous study in which scanning electron micrographs illustrated internalization of the particles. These results suggest that clastogenesis may be a mechanism for lead chromate induced carcinogenesis.

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Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

January 1992

Volume

278

Issue

1

Start / End Page

69 / 79

Location

Netherlands

Related Subject Headings

  • Skin
  • Oncology & Carcinogenesis
  • Mutagens
  • Mutagenicity Tests
  • Lead
  • Karyotyping
  • Humans
  • Cricetinae
  • Chromosome Deletion
  • Chromosome Aberrations
 

Citation

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Wise, J. P., Leonard, J. C., & Patierno, S. R. (1992). Clastogenicity of lead chromate particles in hamster and human cells. Mutat Res, 278(1), 69–79. https://doi.org/10.1016/0165-1218(92)90287-a
Wise, J. P., J. C. Leonard, and S. R. Patierno. “Clastogenicity of lead chromate particles in hamster and human cells.Mutat Res 278, no. 1 (January 1992): 69–79. https://doi.org/10.1016/0165-1218(92)90287-a.
Wise JP, Leonard JC, Patierno SR. Clastogenicity of lead chromate particles in hamster and human cells. Mutat Res. 1992 Jan;278(1):69–79.
Wise, J. P., et al. “Clastogenicity of lead chromate particles in hamster and human cells.Mutat Res, vol. 278, no. 1, Jan. 1992, pp. 69–79. Pubmed, doi:10.1016/0165-1218(92)90287-a.
Wise JP, Leonard JC, Patierno SR. Clastogenicity of lead chromate particles in hamster and human cells. Mutat Res. 1992 Jan;278(1):69–79.
Journal cover image

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

January 1992

Volume

278

Issue

1

Start / End Page

69 / 79

Location

Netherlands

Related Subject Headings

  • Skin
  • Oncology & Carcinogenesis
  • Mutagens
  • Mutagenicity Tests
  • Lead
  • Karyotyping
  • Humans
  • Cricetinae
  • Chromosome Deletion
  • Chromosome Aberrations