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Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1.

Publication ,  Journal Article
Schultz, TF; Spiker, S; Quatrano, RS
Published in: The Journal of biological chemistry
October 1996

Previous work indicated that nuclear extracts isolated from embryogenic rice suspension cells treated with the phytohormone abscisic acid (ABA) have enhanced binding activity to an ABA response element (Em1a) in the promoter of the Em gene from wheat. We identified an activity in wheat and maize nuclear extracts that enhances binding of the recombinant transcription factor EmBP-1 to Em1a by 80-fold. Fractionation of nuclear extracts led us to identify histone H1 and HMGb (but not HMGc or -d) as two factors that can enhance the ability of EmBP-1 to bind to Em1a and account for at least a part of this activity of nuclear extracts. Our results, which indicate for the first time that histone H1 possesses this type of activity, lend further support to the model that positively charged proteins can drastically affect the DNA binding activity of specific transcription factors. Furthermore, our study points to these chromosomal proteins as potential targets of an ABA-mediated modification (e.g. acetylation) that could affect the regulation of Em gene expression.

Duke Scholars

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

October 1996

Volume

271

Issue

42

Start / End Page

25742 / 25745

Related Subject Headings

  • Transcription Factors
  • Recombinant Fusion Proteins
  • Protamines
  • Promoter Regions, Genetic
  • Plant Proteins
  • Leucine Zippers
  • Histones
  • High Mobility Group Proteins
  • Electrophoresis, Polyacrylamide Gel
  • DNA-Binding Proteins
 

Citation

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Schultz, T. F., Spiker, S., & Quatrano, R. S. (1996). Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1. The Journal of Biological Chemistry, 271(42), 25742–25745. https://doi.org/10.1074/jbc.271.42.25742
Schultz, T. F., S. Spiker, and R. S. Quatrano. “Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1.The Journal of Biological Chemistry 271, no. 42 (October 1996): 25742–45. https://doi.org/10.1074/jbc.271.42.25742.
Schultz TF, Spiker S, Quatrano RS. Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1. The Journal of biological chemistry. 1996 Oct;271(42):25742–5.
Schultz, T. F., et al. “Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1.The Journal of Biological Chemistry, vol. 271, no. 42, Oct. 1996, pp. 25742–45. Epmc, doi:10.1074/jbc.271.42.25742.
Schultz TF, Spiker S, Quatrano RS. Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1. The Journal of biological chemistry. 1996 Oct;271(42):25742–25745.

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

October 1996

Volume

271

Issue

42

Start / End Page

25742 / 25745

Related Subject Headings

  • Transcription Factors
  • Recombinant Fusion Proteins
  • Protamines
  • Promoter Regions, Genetic
  • Plant Proteins
  • Leucine Zippers
  • Histones
  • High Mobility Group Proteins
  • Electrophoresis, Polyacrylamide Gel
  • DNA-Binding Proteins