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Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity.

Publication ,  Journal Article
Siddiqui, JF; Van Mater, D; Sowden, MP; Smith, HC
Published in: Exp Cell Res
October 10, 1999

Apolipoprotein B (apoB) mRNA editing is a site-specific (nucleotide 6666) cytidine to uridine transition catalyzed by a cytidine deaminase, APOBEC-1, in the context of a multiprotein complex referred to as the C/U editosome. This report quantifies for the first time the effect of altering APOBEC-1 protein abundance on the proportion of edited apoB mRNAs using transfected McArdle rat hepatoma cells which had been sorted by flow cytometry into populations expressing different levels of green fluorescent protein-APOBEC-1 chimera, GFP-APOBEC. A correlation was observed in which increased expression of GFP-APOBEC protein resulted in a higher proportion of edited apoB mRNA. The number of enzyme molecules required to increase the proportion of edited apoB RNAs was disproportionately high relative to that which might have been predicted from a typical catalytic relationship. Moreover, editing of apoB mRNA at inappropriate sites (promiscuous editing) occurred in response to overexpressing GFP-APOBEC. The data suggest that experimental manipulation of APOBEC-1 abundance in the absence of other regulatory considerations will always result in some level of promiscuous editing. Coordinate expression of APOBEC-1 and the auxiliary proteins and/or regulation of their interactions may be required to increase editing activity without losing editing-site fidelity.

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Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

October 10, 1999

Volume

252

Issue

1

Start / End Page

154 / 164

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Fusion Proteins
  • Rats
  • RNA, Messenger
  • RNA Editing
  • Luminescent Proteins
  • Liver Neoplasms, Experimental
  • Green Fluorescent Proteins
  • Gene Expression
  • DNA Primers
 

Citation

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Siddiqui, J. F., Van Mater, D., Sowden, M. P., & Smith, H. C. (1999). Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity. Exp Cell Res, 252(1), 154–164. https://doi.org/10.1006/excr.1999.4598
Siddiqui, J. F., D. Van Mater, M. P. Sowden, and H. C. Smith. “Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity.Exp Cell Res 252, no. 1 (October 10, 1999): 154–64. https://doi.org/10.1006/excr.1999.4598.
Siddiqui JF, Van Mater D, Sowden MP, Smith HC. Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity. Exp Cell Res. 1999 Oct 10;252(1):154–64.
Siddiqui, J. F., et al. “Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity.Exp Cell Res, vol. 252, no. 1, Oct. 1999, pp. 154–64. Pubmed, doi:10.1006/excr.1999.4598.
Siddiqui JF, Van Mater D, Sowden MP, Smith HC. Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity. Exp Cell Res. 1999 Oct 10;252(1):154–164.
Journal cover image

Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

October 10, 1999

Volume

252

Issue

1

Start / End Page

154 / 164

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Fusion Proteins
  • Rats
  • RNA, Messenger
  • RNA Editing
  • Luminescent Proteins
  • Liver Neoplasms, Experimental
  • Green Fluorescent Proteins
  • Gene Expression
  • DNA Primers