Controlled exposure to diesel exhaust causes increased nitrite in exhaled breath condensate among subjects with asthma.

Journal Article (Journal Article)


To determine whether oxidative/nitrosative stress plays a role in the acute effects of diesel exhaust (DE) on subjects with asthma.


In this crossover study, 16 subjects with mild to moderate asthma were exposed to clean filtered air or diluted DE (300 μg/m as PM2.5) for 1 hour with intermittent exercise.


Airway hyperreactivity increased 24 hours after exposure to DE compared with clean filtered air (PC20, 14.9 mg/mL vs 19.7 mg/mL; P = 0.012). Nitrite in exhaled breath condensate was elevated immediately after diesel exposure (P = 0.052) and remained elevated 4 and 24 hours after exposure.


After exposure to DE, subjects with asthma demonstrated increased airway hyperreactivity and obstruction. Increased nitrite in exhaled breath condensate, in the absence of increased exhaled nitric oxide, suggests a noninflammatory oxidative stress mechanism by which DE affects the lung.

Full Text

Duke Authors

Cited Authors

  • Hussain, S; Laumbach, R; Coleman, J; Youssef, H; Kelly-McNeil, K; Ohman-Strickland, P; Zhang, J; Kipen, H

Published Date

  • October 2012

Published In

Volume / Issue

  • 54 / 10

Start / End Page

  • 1186 - 1191

PubMed ID

  • 23001278

Pubmed Central ID

  • PMC4443752

Electronic International Standard Serial Number (EISSN)

  • 1536-5948

International Standard Serial Number (ISSN)

  • 1076-2752

Digital Object Identifier (DOI)

  • 10.1097/jom.0b013e31826bb64c


  • eng