Different obscurin isoforms localize to distinct sites at sarcomeres.
Journal Article (Journal Article)
We used four antibodies to regions of obscurin isoforms A and B, encoded by the obscurin gene, to investigate the location of these proteins in skeletal myofibers at resting and stretched lengths. Obscurin A ( approximately 800 kDa) which was recognized by antibodies generated to the N-terminal, Rho-GEF, and the non-modular C-terminal domain that lacks the kinase-like domains, localizes at the level of the M-band. Obscurin B ( approximately 900 kDa) which has the N-terminal, Rho-GEF, and the C-terminal kinase-like domains, localizes at the level of the A/I junction. Additional isoforms, which lack one or more of these epitopes, are present at the Z-disk and Z/I junction.
Full Text
Duke Authors
Cited Authors
- Bowman, AL; Kontrogianni-Konstantopoulos, A; Hirsch, SS; Geisler, SB; Gonzalez-Serratos, H; Russell, MW; Bloch, RJ
Published Date
- April 17, 2007
Published In
Volume / Issue
- 581 / 8
Start / End Page
- 1549 - 1554
PubMed ID
- 17382936
Pubmed Central ID
- PMC1899168
International Standard Serial Number (ISSN)
- 0014-5793
Digital Object Identifier (DOI)
- 10.1016/j.febslet.2007.03.011
Language
- eng
Conference Location
- England