Different obscurin isoforms localize to distinct sites at sarcomeres.

Journal Article (Journal Article)

We used four antibodies to regions of obscurin isoforms A and B, encoded by the obscurin gene, to investigate the location of these proteins in skeletal myofibers at resting and stretched lengths. Obscurin A ( approximately 800 kDa) which was recognized by antibodies generated to the N-terminal, Rho-GEF, and the non-modular C-terminal domain that lacks the kinase-like domains, localizes at the level of the M-band. Obscurin B ( approximately 900 kDa) which has the N-terminal, Rho-GEF, and the C-terminal kinase-like domains, localizes at the level of the A/I junction. Additional isoforms, which lack one or more of these epitopes, are present at the Z-disk and Z/I junction.

Full Text

Duke Authors

Cited Authors

  • Bowman, AL; Kontrogianni-Konstantopoulos, A; Hirsch, SS; Geisler, SB; Gonzalez-Serratos, H; Russell, MW; Bloch, RJ

Published Date

  • April 17, 2007

Published In

Volume / Issue

  • 581 / 8

Start / End Page

  • 1549 - 1554

PubMed ID

  • 17382936

Pubmed Central ID

  • PMC1899168

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/j.febslet.2007.03.011

Language

  • eng

Conference Location

  • England