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Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex.

Publication ,  Journal Article
Ursitti, JA; Lee, PC; Resneck, WG; McNally, MM; Bowman, AL; O'Neill, A; Stone, MR; Bloch, RJ
Published in: J Biol Chem
October 1, 2004

We used degenerate primers for the amino- and carboxyl-terminal ends of the rod domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and clone cytokeratins 8 and 19 (K8 and K19) from cardiac muscle of the adult rat. Northern blots showed that K8 has a 2.2-kb transcript and K19 has a 1.9-kb transcript in both adult cardiac and skeletal muscles. Immunolocalization of the cytokeratins in adult cardiac muscle with isoform-specific antibodies for K8 and K19 showed labeling at Z-lines within the muscle fibers and at Z-line and M-line domains at costameres at the sarcolemmal membrane. Dystrophin and K19 could be co-immunoprecipitated and co-purified from extracts of cardiac muscle, suggesting a link between the cytokeratins and the dystrophin-based cytoskeleton at the sarcolemma. Furthermore, transfection experiments indicate that K8 and K19 may associate with dystrophin through a specific interaction with its actin-binding domain. Consistent with this observation, the cytokeratins are disrupted at the sarcolemmal membrane of skeletal muscle of the mdx mouse that lacks dystrophin. Together these results indicate that at least two cytokeratins are expressed in adult striated muscle, where they may contribute to the organization of both the myoplasm and sarcolemma.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

October 1, 2004

Volume

279

Issue

40

Start / End Page

41830 / 41838

Location

United States

Related Subject Headings

  • Sarcolemma
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Binding
  • Myocardium
  • Muscle, Skeletal
  • Keratins
  • Immunohistochemistry
  • Dystrophin
 

Citation

APA
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ICMJE
MLA
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Ursitti, J. A., Lee, P. C., Resneck, W. G., McNally, M. M., Bowman, A. L., O’Neill, A., … Bloch, R. J. (2004). Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex. J Biol Chem, 279(40), 41830–41838. https://doi.org/10.1074/jbc.M400128200
Ursitti, Jeanine A., Pervis C. Lee, Wendy G. Resneck, Minda M. McNally, Amber L. Bowman, Andrea O’Neill, Michele R. Stone, and Robert J. Bloch. “Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex.J Biol Chem 279, no. 40 (October 1, 2004): 41830–38. https://doi.org/10.1074/jbc.M400128200.
Ursitti JA, Lee PC, Resneck WG, McNally MM, Bowman AL, O’Neill A, et al. Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex. J Biol Chem. 2004 Oct 1;279(40):41830–8.
Ursitti, Jeanine A., et al. “Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex.J Biol Chem, vol. 279, no. 40, Oct. 2004, pp. 41830–38. Pubmed, doi:10.1074/jbc.M400128200.
Ursitti JA, Lee PC, Resneck WG, McNally MM, Bowman AL, O’Neill A, Stone MR, Bloch RJ. Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex. J Biol Chem. 2004 Oct 1;279(40):41830–41838.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

October 1, 2004

Volume

279

Issue

40

Start / End Page

41830 / 41838

Location

United States

Related Subject Headings

  • Sarcolemma
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Binding
  • Myocardium
  • Muscle, Skeletal
  • Keratins
  • Immunohistochemistry
  • Dystrophin