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Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury.

Publication ,  Journal Article
Roberts, J; Chen, B; Curtis, LM; Agarwal, A; Sanders, PW; Zinn, KR
Published in: Am J Physiol Renal Physiol
October 2007

Accurate determination of renal function in mice is a major impediment to the use of murine models in acute kidney injury. The purpose of this study was to determine whether early changes in renal function could be detected using dynamic gamma camera imaging in a mouse model of ischemia-reperfusion (I/R) injury. C57BL/6 mice (n = 5/group) underwent a right nephrectomy, followed by either 30 min of I/R injury or sham surgery of the remaining kidney. Dynamic renal studies (21 min, 10 s/frame) were conducted before surgery (baseline) and at 5, 24, and 48 h by injection of (99m)Tc-mercaptoacetyltriglycine (MAG3; approximately 1.0 mCi/mouse) via the tail vein. The percentage of injected dose (%ID) in the kidney was calculated for each 10-s interval after MAG3 injection, using standard region of interest analyses. A defect in renal function in I/R-treated mice was detected as early as 5 h after surgery compared with sham-treated mice, identified by the increased %ID (at peak) in the I/R-treated kidneys at 100 s (P < 0.01) that remained significantly higher than sham-treated mice for the duration of the scan until 600 s (P < 0.05). At 48 h, the renal scan demonstrated functional renal recovery of the I/R mice and was comparable to sham-treated mice. Our study shows that using dynamic imaging, renal dysfunction can be detected and quantified reliably as early as 5 h after I/R insult, allowing for evaluation of early treatment interventions.

Duke Scholars

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

October 2007

Volume

293

Issue

4

Start / End Page

F1408 / F1412

Location

United States

Related Subject Headings

  • Whole Body Imaging
  • Urology & Nephrology
  • Technetium Tc 99m Mertiatide
  • Reperfusion Injury
  • Radiopharmaceuticals
  • Radionuclide Imaging
  • Oncogene Proteins
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Roberts, J., Chen, B., Curtis, L. M., Agarwal, A., Sanders, P. W., & Zinn, K. R. (2007). Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury. Am J Physiol Renal Physiol, 293(4), F1408–F1412. https://doi.org/10.1152/ajprenal.00083.2007
Roberts, John, Bo Chen, Lisa M. Curtis, Anupam Agarwal, Paul W. Sanders, and Kurt R. Zinn. “Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury.Am J Physiol Renal Physiol 293, no. 4 (October 2007): F1408–12. https://doi.org/10.1152/ajprenal.00083.2007.
Roberts J, Chen B, Curtis LM, Agarwal A, Sanders PW, Zinn KR. Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2007 Oct;293(4):F1408–12.
Roberts, John, et al. “Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury.Am J Physiol Renal Physiol, vol. 293, no. 4, Oct. 2007, pp. F1408–12. Pubmed, doi:10.1152/ajprenal.00083.2007.
Roberts J, Chen B, Curtis LM, Agarwal A, Sanders PW, Zinn KR. Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2007 Oct;293(4):F1408–F1412.

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

October 2007

Volume

293

Issue

4

Start / End Page

F1408 / F1412

Location

United States

Related Subject Headings

  • Whole Body Imaging
  • Urology & Nephrology
  • Technetium Tc 99m Mertiatide
  • Reperfusion Injury
  • Radiopharmaceuticals
  • Radionuclide Imaging
  • Oncogene Proteins
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins