Mice with a deletion in the gene for CCAAT/enhancer-binding protein beta are protected against diet-induced obesity.

Published

Journal Article

The CCAAT/enhancer-binding protein beta (C/EBPbeta) is required for adipocyte differentiation and maturation. We have studied the role of the transcription factor, C/EBPbeta, in the development of diet-induced obesity. Mice with a deletion in the gene for C/EBPbeta (C/EBPbeta(-/-)) and wild-type mice were fed a high-fat diet (60% fat) for 12 weeks. The C/EBPbeta(-/-) mice lost body fat, whereas the wild-type mice increased their total body fat on a high-fat diet. The C/EBPbeta(-/-) mice had lower levels of blood triglycerides, free fatty acids, cholesterol, and hepatic triglyceride accumulation compared with the wild-type mice, thus protecting them from diet-induced obesity and fatty liver on a high-fat diet. Deletion of C/EBPbeta gene resulted in greatly reducing hepatic lipogenic genes, acetyl CoA carboxylase, and fatty acid synthase and increasing the expression of beta-oxidation genes in the brown adipose tissue. CO(2) production was significantly higher in the C/EBPbeta(-/-) mice as was the level of uncoupling protein (UCP)-1 and UCP-3 in the muscle. In conclusion, the transcription factor C/EBPbeta is an important regulator in controlling lipid metabolism and in the development of diet-induced obesity.

Full Text

Duke Authors

Cited Authors

  • Millward, CA; Heaney, JD; Sinasac, DS; Chu, EC; Bederman, IR; Gilge, DA; Previs, SF; Croniger, CM

Published Date

  • January 1, 2007

Published In

Volume / Issue

  • 56 / 1

Start / End Page

  • 161 - 167

PubMed ID

  • 17192478

Pubmed Central ID

  • 17192478

International Standard Serial Number (ISSN)

  • 0012-1797

Digital Object Identifier (DOI)

  • 10.2337/db06-0310

Language

  • eng

Conference Location

  • United States