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Identification of candidate genes in ulcerative colitis and Crohn's disease using cDNA array technology.

Publication ,  Journal Article
Uthoff, SM; Eichenberger, MR; Lewis, RK; Fox, MP; Hamilton, CJ; McAuliffe, TL; Grimes, HL; Galandiuk, S
Published in: Int J Oncol
October 2001

Inflammatory bowel disease (IBD) follows a multigenic mode of inheritance, encompassing the clinically discrete phenotypes of ulcerative colitis (UC) and Crohn's disease (CD). The risk of malignant transformation of the colon increases with the duration and extent of IBD and is particularly high for patients with a longstanding history of UC. We wished to identify candidate genes that might be involved in disease pathogenesis based on functional plausibility and their putative role in IBD carcinogenesis. Polyadenylated mRNA (PolyA+ mRNA) preparation from inflamed intestinal mucosa of patients with a longstanding history of UC and CD was performed with subsequent hybridization of alpha phosphorus [alpha-32P]-deoxyadenotriphosphate-labeled complementary deoxyribonucleic acid (DNA) populations to nucleic acid arrays. Of 588 different human gene transcripts arrayed, secreted apoptosis-related protein 1 (Sarp1), frizzled (fz) homologues, and disheveled (dvl) were differentially expressed, being elevated in UC as compared to CD. These genes encode proteins involved in the Wingless-type (Wnt)/beta-catenin signaling pathway. The autonomous expression of Sarp1 and Sarp1-compatible fz receptor genes suggests that the Wnt pathway may be involved in UC carcinogenesis.

Duke Scholars

Published In

Int J Oncol

DOI

ISSN

1019-6439

Publication Date

October 2001

Volume

19

Issue

4

Start / End Page

803 / 810

Location

Greece

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Membrane Proteins
  • Inflammation
  • Humans
  • Gene Expression Profiling
  • DNA, Neoplasm
  • DNA Primers
  • Crohn Disease
 

Citation

APA
Chicago
ICMJE
MLA
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Uthoff, S. M., Eichenberger, M. R., Lewis, R. K., Fox, M. P., Hamilton, C. J., McAuliffe, T. L., … Galandiuk, S. (2001). Identification of candidate genes in ulcerative colitis and Crohn's disease using cDNA array technology. Int J Oncol, 19(4), 803–810. https://doi.org/10.3892/ijo.19.4.803
Uthoff, S. M., M. R. Eichenberger, R. K. Lewis, M. P. Fox, C. J. Hamilton, T. L. McAuliffe, H. L. Grimes, and S. Galandiuk. “Identification of candidate genes in ulcerative colitis and Crohn's disease using cDNA array technology.Int J Oncol 19, no. 4 (October 2001): 803–10. https://doi.org/10.3892/ijo.19.4.803.
Uthoff SM, Eichenberger MR, Lewis RK, Fox MP, Hamilton CJ, McAuliffe TL, et al. Identification of candidate genes in ulcerative colitis and Crohn's disease using cDNA array technology. Int J Oncol. 2001 Oct;19(4):803–10.
Uthoff, S. M., et al. “Identification of candidate genes in ulcerative colitis and Crohn's disease using cDNA array technology.Int J Oncol, vol. 19, no. 4, Oct. 2001, pp. 803–10. Pubmed, doi:10.3892/ijo.19.4.803.
Uthoff SM, Eichenberger MR, Lewis RK, Fox MP, Hamilton CJ, McAuliffe TL, Grimes HL, Galandiuk S. Identification of candidate genes in ulcerative colitis and Crohn's disease using cDNA array technology. Int J Oncol. 2001 Oct;19(4):803–810.

Published In

Int J Oncol

DOI

ISSN

1019-6439

Publication Date

October 2001

Volume

19

Issue

4

Start / End Page

803 / 810

Location

Greece

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Membrane Proteins
  • Inflammation
  • Humans
  • Gene Expression Profiling
  • DNA, Neoplasm
  • DNA Primers
  • Crohn Disease