Temporal and molecular separation of the kit receptor tyrosine kinase's roles in zebrafish melanocyte migration and survival.

Journal Article (Journal Article)

The Kit receptor tyrosine kinase is required by vertebrate melanocytes for their migration and survival. The relationship between these developmental roles of Kit, however, remains poorly understood. Here, we use two genetic approaches to demonstrate that Kit's roles in the migration and survival of embryonic melanocytes in the zebrafish (Danio rerio) are temporally and functionally independent. We use a temperature-sensitive kit mutation to show that kit promotes melanocyte migration and survival during distinct stages of development. These experiments additionally reveal that melanocyte migration is neither necessary nor sufficient for subsequent survival. We also identify kit alleles that molecularly separate kits roles in migration and survival. These results suggest that the melanocyte changes its response to Kit receptor signaling and function during development, first to promote migration, then to promote survival through distinct Kit-dependent mechanisms.

Full Text

Duke Authors

Cited Authors

  • Rawls, JF; Johnson, SL

Published Date

  • October 1, 2003

Published In

Volume / Issue

  • 262 / 1

Start / End Page

  • 152 - 161

PubMed ID

  • 14512025

International Standard Serial Number (ISSN)

  • 0012-1606

Digital Object Identifier (DOI)

  • 10.1016/s0012-1606(03)00386-5


  • eng

Conference Location

  • United States