Bone marrow B cell precursor number after allogeneic stem cell transplantation and GVHD development.

Journal Article (Journal Article)

Patients without chronic graft-versus-host disease (cGVHD) have robust B cell reconstitution and are able to maintain B cell homeostasis after allogeneic hematopoietic stem cell transplantation (HSCT). To determine whether B lymphopoiesis differs before cGVHD develops, we examined bone marrow (BM) biopsies for terminal deoxynucleotidyl transferase (TdT) and PAX5 immunostaining early post-HSCT at day 30 when all patients have been shown to have high B cell activating factor (BAFF) levels. We found significantly greater numbers of BM B cell precursors in patients who did not develop cGVHD compared with those who developed cGVHD (median = 44 vs 2 cells/high powered field [hpf]; respectively; P < .001). Importantly, a significant increase in precursor B cells was maintained when patients receiving high-dose steroid therapy were excluded (median = 49 vs 20 cells/hpf; P = .017). Thus, we demonstrate the association of BM B cell production capacity in human GVHD development. Increased BM precursor B cell number may serve to predict good clinical outcome after HSCT.

Full Text

Duke Authors

Cited Authors

  • Fedoriw, Y; Samulski, TD; Deal, AM; Dunphy, CH; Sharf, A; Shea, TC; Serody, JS; Sarantopoulos, S

Published Date

  • June 2012

Published In

Volume / Issue

  • 18 / 6

Start / End Page

  • 968 - 973

PubMed ID

  • 22446015

Pubmed Central ID

  • PMC3693471

Electronic International Standard Serial Number (EISSN)

  • 1523-6536

Digital Object Identifier (DOI)

  • 10.1016/j.bbmt.2012.03.005


  • eng

Conference Location

  • United States