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Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation.

Publication ,  Journal Article
Porcheray, F; Miklos, DB; Floyd, BH; Sarantopoulos, S; Bellucci, R; Soiffer, RJ; Antin, JH; Alyea, EP; Ritz, J; Zorn, E
Published in: Transplantation
August 15, 2011

BACKGROUND: Antibody responses to HY antigens in male recipients are frequent after transplantation of stem cells from female donors (Miklos et al., Blood 2005; 105: 2973; Miklos et al., Blood 2004; 103: 353). However, evidence that this B-cell immunity is accompanied by T-cell responses to the cognate antigens is scarce. Here, we examined T- and B-cell responses to DBY antigen in a male patient who received hematopoietic stem cells from a human leukocyte antigen-identical female sibling. MATERIALS AND METHODS: We used 93 overlapping peptides representing the entire DBY protein to detect and characterize T-cell and antibody responses to DBY by enzyme-linked immunosorbent spot (ELISPOT) and enzyme-linked immunosorbent assay. RESULTS: High frequency CD4+ T cells specific for a unique DBY peptide were detected in the patient blood. We isolated the corresponding T-cell clone and characterized the recognized epitope as an 18-mer peptide restricted by human leukocyte antigen-DRB1*0101. Upon stimulation, this clone produced cytokines with no evidence of Th1 or Th2 polarization. Remarkably, this clone also recognized the DBX homologue peptide and responded to female donor dendritic cells stimulated with poly I/C or lipopolysaccharide, indicating that the peptide was endogenously processed in these cells. High titer DBY-specific antibodies were also found in the patient serum which, in contrast to the T-cell response, did not cross-react with DBX. CONCLUSION: We show here the development of a coordinated B and T-cell response to DBY in a recipient of sex mismatched allogeneic hematopoietic stem-cell transplantation. Our findings support a role for CD4+ T cells in the development of humoral immunity to minor histocompatibility antigens.

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Published In

Transplantation

DOI

EISSN

1534-6080

Publication Date

August 15, 2011

Volume

92

Issue

3

Start / End Page

359 / 365

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Surgery
  • Stem Cell Transplantation
  • Sex Factors
  • Minor Histocompatibility Antigens
  • Middle Aged
  • Male
  • Isoantibodies
  • Humans
  • Histocompatibility Testing
 

Citation

APA
Chicago
ICMJE
MLA
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Porcheray, F., Miklos, D. B., Floyd, B. H., Sarantopoulos, S., Bellucci, R., Soiffer, R. J., … Zorn, E. (2011). Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation. Transplantation, 92(3), 359–365. https://doi.org/10.1097/TP.0b013e3182244cc3
Porcheray, Fabrice, David B. Miklos, Blair H. Floyd, Stefanie Sarantopoulos, Roberto Bellucci, Robert J. Soiffer, Joseph H. Antin, Edwin P. Alyea, Jerome Ritz, and Emmanuel Zorn. “Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation.Transplantation 92, no. 3 (August 15, 2011): 359–65. https://doi.org/10.1097/TP.0b013e3182244cc3.
Porcheray F, Miklos DB, Floyd BH, Sarantopoulos S, Bellucci R, Soiffer RJ, et al. Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation. Transplantation. 2011 Aug 15;92(3):359–65.
Porcheray, Fabrice, et al. “Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation.Transplantation, vol. 92, no. 3, Aug. 2011, pp. 359–65. Pubmed, doi:10.1097/TP.0b013e3182244cc3.
Porcheray F, Miklos DB, Floyd BH, Sarantopoulos S, Bellucci R, Soiffer RJ, Antin JH, Alyea EP, Ritz J, Zorn E. Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation. Transplantation. 2011 Aug 15;92(3):359–365.

Published In

Transplantation

DOI

EISSN

1534-6080

Publication Date

August 15, 2011

Volume

92

Issue

3

Start / End Page

359 / 365

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Surgery
  • Stem Cell Transplantation
  • Sex Factors
  • Minor Histocompatibility Antigens
  • Middle Aged
  • Male
  • Isoantibodies
  • Humans
  • Histocompatibility Testing