Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum.
We had developed a technology for creation of recombinant polyclonal antibody libraries, standardized perpetual mixtures of polyclonal whole antibodies for which the genes are available and can be altered as desired. We report here the first phase of generating a polyclonal antibody library to Cryptosporidium parvum, a protozoan parasite that causes severe disease in AIDS patients, for which there is no effective treatment. BALB/c mice, immunized by neonatal oral infection with oocysts followed by intraperitoneal immunization with a sporozoite/oocyst preparation of C. parvum, were used for construction of a Fab phage display library in a specially-designed bidirectional vector. This library was selected for reactivity to an oocyst/sporozoite preparation, and was shown to be antigen-specific and diverse. Following mass transfer of the selected variable region gene pairs to appropriate mammalian expression vectors, such anti-C. parvum Fab phage display libraries could be used to develop chimeric polyclonal antibody libraries, with mouse variable regions and human constant regions, for passive immunotherapy of C. parvum infection.
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Related Subject Headings
- Peptide Library
- Organic Chemistry
- Molecular Sequence Data
- Mice, Inbred BALB C
- Mice
- Immunoglobulin Variable Region
- Immunoglobulin Light Chains
- Immunoglobulin Heavy Chains
- Immunoglobulin Fab Fragments
- Humans
Citation
Published In
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Peptide Library
- Organic Chemistry
- Molecular Sequence Data
- Mice, Inbred BALB C
- Mice
- Immunoglobulin Variable Region
- Immunoglobulin Light Chains
- Immunoglobulin Heavy Chains
- Immunoglobulin Fab Fragments
- Humans