Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum.

Published

Journal Article

We had developed a technology for creation of recombinant polyclonal antibody libraries, standardized perpetual mixtures of polyclonal whole antibodies for which the genes are available and can be altered as desired. We report here the first phase of generating a polyclonal antibody library to Cryptosporidium parvum, a protozoan parasite that causes severe disease in AIDS patients, for which there is no effective treatment. BALB/c mice, immunized by neonatal oral infection with oocysts followed by intraperitoneal immunization with a sporozoite/oocyst preparation of C. parvum, were used for construction of a Fab phage display library in a specially-designed bidirectional vector. This library was selected for reactivity to an oocyst/sporozoite preparation, and was shown to be antigen-specific and diverse. Following mass transfer of the selected variable region gene pairs to appropriate mammalian expression vectors, such anti-C. parvum Fab phage display libraries could be used to develop chimeric polyclonal antibody libraries, with mouse variable regions and human constant regions, for passive immunotherapy of C. parvum infection.

Full Text

Duke Authors

Cited Authors

  • Baecher-Allan, CM; Santora, K; Sarantopoulos, S; Den, W; Sompuram, SR; Sharon, J; Cevallos, AM; Bhat, N; Ward, H

Published Date

  • December 1999

Published In

Volume / Issue

  • 2 / 6

Start / End Page

  • 319 - 325

PubMed ID

  • 10644857

Pubmed Central ID

  • 10644857

Electronic International Standard Serial Number (EISSN)

  • 1875-5402

International Standard Serial Number (ISSN)

  • 1386-2073

Language

  • eng