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In vitro benzo[a]pyrene diol epoxide-induced DNA damage and chromosomal aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck.

Publication ,  Journal Article
Xiong, P; Hu, Z; Li, C; Wang, L-E; El-Naggar, AK; Sturgis, EM; Wei, Q
Published in: Int J Cancer
December 15, 2007

Cigarette smoking is a major risk factor for squamous cell carcinoma of the head and neck (SCCHN), but only a fraction of those exposed to cigarette smoke develops SCCHN, suggesting variation in individual susceptibility. Tobacco smoke contains a number of carcinogens that cause various kinds of damage to DNA. In this study, we simultaneously measured benzo[a]pyrene diol epoxide-induced DNA damage and chromosomal aberrations by the comet assay and the mutagen sensitivity assay, respectively, in cultured primary lymphocytes from newly recruited 123 patients with SCCHN and 136 age- and sex-matched controls. Using the control median as the cut-off, the elevated risk of SCCHN was 2.35 (95% CI, 1.37-4.03), 2.28 (95% CI, 1.34-3.98) and 3.25 (95% CI, 1.85-5.07) for high levels of tail extension, tail length and oliver tail moment of the comet assay, respectively, and 1.75 (95% CI, 1.04-2.94) for high levels of chromosomal aberrations of the mutagen sensitivity assay. The effects of these 2 types of measurements were additive; subjects with high levels of both DNA damage and chromosomal aberrations had a 4.77-fold increased risk (95% CI, 2.73-8.36) of SCCHN. Cigarette smoking further elevated this risk to more than 20-fold (OR 23.6; 95% CI, 8.92-62.3). These data support our previous finding that suboptimal repair contributed to susceptibility to SCCHN and the new data further suggests a possible gene-environment interaction that may play an important role in the etiology of SCCHN. Further validation studies are warranted.

Duke Scholars

Published In

Int J Cancer

DOI

EISSN

1097-0215

Publication Date

December 15, 2007

Volume

121

Issue

12

Start / End Page

2735 / 2740

Location

United States

Related Subject Headings

  • Texas
  • Smoking
  • Risk Factors
  • Risk Assessment
  • Oncology & Carcinogenesis
  • Odds Ratio
  • Mutagens
  • Middle Aged
  • Male
  • Lymphocytes
 

Citation

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ICMJE
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Xiong, P., Hu, Z., Li, C., Wang, L.-E., El-Naggar, A. K., Sturgis, E. M., & Wei, Q. (2007). In vitro benzo[a]pyrene diol epoxide-induced DNA damage and chromosomal aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck. Int J Cancer, 121(12), 2735–2740. https://doi.org/10.1002/ijc.23054
Xiong, Ping, Zhibin Hu, Chunying Li, Li-E Wang, Adel K. El-Naggar, Erich M. Sturgis, and Qingyi Wei. “In vitro benzo[a]pyrene diol epoxide-induced DNA damage and chromosomal aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck.Int J Cancer 121, no. 12 (December 15, 2007): 2735–40. https://doi.org/10.1002/ijc.23054.
Journal cover image

Published In

Int J Cancer

DOI

EISSN

1097-0215

Publication Date

December 15, 2007

Volume

121

Issue

12

Start / End Page

2735 / 2740

Location

United States

Related Subject Headings

  • Texas
  • Smoking
  • Risk Factors
  • Risk Assessment
  • Oncology & Carcinogenesis
  • Odds Ratio
  • Mutagens
  • Middle Aged
  • Male
  • Lymphocytes