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Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells.

Publication ,  Journal Article
Gong, Z; Yang, J; Yang, M; Wang, F; Wei, Q; Tanguay, RM; Wu, T
Published in: Toxicol Lett
October 25, 2006

Benzo(a)pyrene (BaP), a ubiquitous environmental pollutant known to cause many diseases including atherosclerosis, induces a dose-dependent reduction in the levels of the inducible Hsp70. To explore the mechanism underlying the reduction of Hsp70, we measured the levels of Hsp70, cytoplasmic and nuclear heat shock factor 1 (HSF1) in porcine aortic endothelial cells using Western blot, and then further characterized the binding ability of HSF1 and heat shock element (HSE) by electrophoretic mobility shift assay. We found that when porcine aortic endothelial cells were treated by 0.1-10 microM of BaP for 24 h, there was a significant reduction of Hsp70, cytoplasmic and nuclear HSF1 and the binding rate of HSF1 and HSE at 5, 10 microM of BaP but less effective at lower concentrations. The effect of BaP on the Hsp70 expression level was markedly attenuated by co-treatment with phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC). Staurosporine (STP), an inhibitor of PKC, blocked the effect of PMA treatment in combination with BaP. These results suggest that BaP might inhibit Hsp70 levels by reducing the expression of HSF1 and decreasing binding of HSF1 and HSE via PKC-dependent signaling pathways that might be involved in the regulation of Hsp70 gene expression under BaP.

Duke Scholars

Published In

Toxicol Lett

DOI

ISSN

0378-4274

Publication Date

October 25, 2006

Volume

166

Issue

3

Start / End Page

229 / 236

Location

Netherlands

Related Subject Headings

  • Toxicology
  • Tetradecanoylphorbol Acetate
  • Swine
  • Staurosporine
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Protein Kinase C
  • Phosphorylation
  • HSP70 Heat-Shock Proteins
  • Enzyme Inhibitors
 

Citation

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Gong, Z., Yang, J., Yang, M., Wang, F., Wei, Q., Tanguay, R. M., & Wu, T. (2006). Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells. Toxicol Lett, 166(3), 229–236. https://doi.org/10.1016/j.toxlet.2006.07.307
Gong, Z., J. Yang, M. Yang, F. Wang, Q. Wei, R. M. Tanguay, and T. Wu. “Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells.Toxicol Lett 166, no. 3 (October 25, 2006): 229–36. https://doi.org/10.1016/j.toxlet.2006.07.307.
Gong Z, Yang J, Yang M, Wang F, Wei Q, Tanguay RM, et al. Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells. Toxicol Lett. 2006 Oct 25;166(3):229–36.
Gong, Z., et al. “Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells.Toxicol Lett, vol. 166, no. 3, Oct. 2006, pp. 229–36. Pubmed, doi:10.1016/j.toxlet.2006.07.307.
Gong Z, Yang J, Yang M, Wang F, Wei Q, Tanguay RM, Wu T. Benzo(a)pyrene inhibits expression of inducible heat shock protein 70 in vascular endothelial cells. Toxicol Lett. 2006 Oct 25;166(3):229–236.
Journal cover image

Published In

Toxicol Lett

DOI

ISSN

0378-4274

Publication Date

October 25, 2006

Volume

166

Issue

3

Start / End Page

229 / 236

Location

Netherlands

Related Subject Headings

  • Toxicology
  • Tetradecanoylphorbol Acetate
  • Swine
  • Staurosporine
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Protein Kinase C
  • Phosphorylation
  • HSP70 Heat-Shock Proteins
  • Enzyme Inhibitors