Skip to main content
Journal cover image

Reliability of mutagen sensitivity assay: an inter-laboratory comparison.

Publication ,  Journal Article
Erdei, E; Lee, S-J; Wei, Q; Wang, L-E; Song, Y-S; Bovbjerg, D; Berwick, M
Published in: Mutagenesis
July 2006

Mutagen sensitivity is regarded as a genetic susceptibility phenotype for various cancers; it is cytogenetically based and probably involves a number of genes from different DNA repair pathways. This assay has been used in a number of laboratories in the field of epidemiology, where it has been investigated and appears to be a useful susceptibility biomarker for epidemiological studies assessing cancer risks at the population level. One concern about phenotypic assays, such as the mutagen sensitivity assay, has been that there could be wide variation in results depending on the timing of the assay (within individual variation), the individual performing the assay (within observer variation) and the laboratory where the assay has been performed (inter-laboratory variation). We conducted an inter-laboratory comparison study between the Memorial Sloan-Kettering Cancer Center and M. D. Anderson, in which we assessed all these concerns. We did not find any significant variation in any of the assays. The correlation was high for all tests. The good concordance rate between laboratories supports the continued use of the mutagen sensitivity assay by different laboratories, and demonstrates its potential to identify at-risk subgroups among normal individuals and cancer patients alike.

Duke Scholars

Published In

Mutagenesis

DOI

ISSN

0267-8357

Publication Date

July 2006

Volume

21

Issue

4

Start / End Page

261 / 264

Location

England

Related Subject Headings

  • Toxicology
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Phenotype
  • Observer Variation
  • Mutagens
  • Mutagenicity Tests
  • Mutagenesis
  • Metaphase
  • Laboratories
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Erdei, E., Lee, S.-J., Wei, Q., Wang, L.-E., Song, Y.-S., Bovbjerg, D., & Berwick, M. (2006). Reliability of mutagen sensitivity assay: an inter-laboratory comparison. Mutagenesis, 21(4), 261–264. https://doi.org/10.1093/mutage/gel030
Erdei, Esther, Sang-Joon Lee, Qingyi Wei, Li-E Wang, Yan-S Song, Dana Bovbjerg, and Marianne Berwick. “Reliability of mutagen sensitivity assay: an inter-laboratory comparison.Mutagenesis 21, no. 4 (July 2006): 261–64. https://doi.org/10.1093/mutage/gel030.
Erdei E, Lee S-J, Wei Q, Wang L-E, Song Y-S, Bovbjerg D, et al. Reliability of mutagen sensitivity assay: an inter-laboratory comparison. Mutagenesis. 2006 Jul;21(4):261–4.
Erdei, Esther, et al. “Reliability of mutagen sensitivity assay: an inter-laboratory comparison.Mutagenesis, vol. 21, no. 4, July 2006, pp. 261–64. Pubmed, doi:10.1093/mutage/gel030.
Erdei E, Lee S-J, Wei Q, Wang L-E, Song Y-S, Bovbjerg D, Berwick M. Reliability of mutagen sensitivity assay: an inter-laboratory comparison. Mutagenesis. 2006 Jul;21(4):261–264.
Journal cover image

Published In

Mutagenesis

DOI

ISSN

0267-8357

Publication Date

July 2006

Volume

21

Issue

4

Start / End Page

261 / 264

Location

England

Related Subject Headings

  • Toxicology
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Phenotype
  • Observer Variation
  • Mutagens
  • Mutagenicity Tests
  • Mutagenesis
  • Metaphase
  • Laboratories