Reliability of mutagen sensitivity assay: an inter-laboratory comparison.

Journal Article

Mutagen sensitivity is regarded as a genetic susceptibility phenotype for various cancers; it is cytogenetically based and probably involves a number of genes from different DNA repair pathways. This assay has been used in a number of laboratories in the field of epidemiology, where it has been investigated and appears to be a useful susceptibility biomarker for epidemiological studies assessing cancer risks at the population level. One concern about phenotypic assays, such as the mutagen sensitivity assay, has been that there could be wide variation in results depending on the timing of the assay (within individual variation), the individual performing the assay (within observer variation) and the laboratory where the assay has been performed (inter-laboratory variation). We conducted an inter-laboratory comparison study between the Memorial Sloan-Kettering Cancer Center and M. D. Anderson, in which we assessed all these concerns. We did not find any significant variation in any of the assays. The correlation was high for all tests. The good concordance rate between laboratories supports the continued use of the mutagen sensitivity assay by different laboratories, and demonstrates its potential to identify at-risk subgroups among normal individuals and cancer patients alike.

Full Text

Duke Authors

Cited Authors

  • Erdei, E; Lee, S-J; Wei, Q; Wang, L-E; Song, Y-S; Bovbjerg, D; Berwick, M

Published Date

  • July 26, 2006

Published In

Volume / Issue

  • 21 / 4

Start / End Page

  • 261 - 264

PubMed ID

  • 16870697

Electronic International Standard Serial Number (EISSN)

  • 1464-3804

International Standard Serial Number (ISSN)

  • 0267-8357

Digital Object Identifier (DOI)

  • 10.1093/mutage/gel030

Language

  • eng