Genomic instability and endoreduplication triggered by RAD17 deletion.

Journal Article

Cell cycle checkpoints are critical for genomic stability. Rad17, a component of the checkpoint clamp loader complex (Rad17/Rfc2-5), is required for the response to DNA damage and replication stress. To explore the role of Rad17 in the maintenance of genomic integrity, we established somatic conditional alleles of RAD17 in human cells. We find that RAD17 is not only important for the Atr-mediated checkpoint but is also essential for cell viability. Cells lacking RAD17 exhibited acute chromosomal aberrations and underwent endoreduplication at a high rate. Therefore, RAD17 links the checkpoint to ploidy control and is essential for the maintenance of chromosomal stability.

Full Text

Duke Authors

Cited Authors

  • Wang, X; Zou, L; Zheng, H; Wei, Q; Elledge, SJ; Li, L

Published Date

  • April 2, 2003

Published In

Volume / Issue

  • 17 / 8

Start / End Page

  • 965 - 970

PubMed ID

  • 12672690

Electronic International Standard Serial Number (EISSN)

  • 1549-5477

International Standard Serial Number (ISSN)

  • 0890-9369

Digital Object Identifier (DOI)

  • 10.1101/gad.1065103

Language

  • eng