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Reduced expression levels of nucleotide excision repair genes in lung cancer: a case-control analysis.

Publication ,  Journal Article
Cheng, L; Spitz, MR; Hong, WK; Wei, Q
Published in: Carcinogenesis
August 2000

Epidemiological studies have indicated that reduced DNA repair capacity and increased DNA adduct levels are associated with increased risk of lung cancer. Nucleotide excision repair (NER) is the major pathway in humans for repairing DNA adducts induced by smoking-related carcinogens, such as benzo[a]pyrene diol epoxide. We hypothesized that genetically determined baseline expression level of genes involved in NER is associated with risk of lung cancer. In a pilot case-control study, we measured the relative expression levels of five NER genes [ERCC1, XPB/ERCC3, XPG/ERCC5, CSB/ERCC6 and XPC (ERCC, excision repair cross-complementing; CSB, Cockayne's syndrome complementary group B)] in phytohemagglutinin-stimulated peripheral lymphocytes obtained from 75 lung cancer patients and 95 controls using a newly developed multiplex RT-PCR assay. Cases and controls were matched on age, sex, ethnicity and tobacco use. The expression level of the beta-actin gene was used as an internal control for the relative quantitation. We observed a 12.2 and 12.5% decrease in the baseline expression levels of XPG/ERCC5 and CSB/ERCC6, respectively, in cases compared with controls. These differences were statistically significant (P < 0.01) when the median expression level in the controls was used as the cut-off point, the lung cancer patients were significantly more likely than the controls to have reduced expression levels of XPG/ERCC5 [odds ratio (OR), 2.32; 95% confidence interval (CI), 1.22-4.43] and CSB/ERCC6 (OR, 2.49; 95% CI, 1.28-4.84). There was also a dose-response relationship between reduced expression levels and increased lung cancer risk (trend test: P < 0.01). Our results suggest that individuals whose expression levels of XPG/ERCC5 and CSB/ERCC6 are reduced may be at higher risk of lung cancer.

Duke Scholars

Published In

Carcinogenesis

ISSN

0143-3334

Publication Date

August 2000

Volume

21

Issue

8

Start / End Page

1527 / 1530

Location

England

Related Subject Headings

  • Transcription Factors
  • Risk Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regression Analysis
  • Proteins
  • Protein Biosynthesis
  • Poly-ADP-Ribose Binding Proteins
  • Pilot Projects
  • Phytohemagglutinins
  • Oncology & Carcinogenesis
 

Citation

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MLA
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Cheng, L., Spitz, M. R., Hong, W. K., & Wei, Q. (2000). Reduced expression levels of nucleotide excision repair genes in lung cancer: a case-control analysis. Carcinogenesis, 21(8), 1527–1530.
Cheng, L., M. R. Spitz, W. K. Hong, and Q. Wei. “Reduced expression levels of nucleotide excision repair genes in lung cancer: a case-control analysis.Carcinogenesis 21, no. 8 (August 2000): 1527–30.
Cheng L, Spitz MR, Hong WK, Wei Q. Reduced expression levels of nucleotide excision repair genes in lung cancer: a case-control analysis. Carcinogenesis. 2000 Aug;21(8):1527–30.
Cheng, L., et al. “Reduced expression levels of nucleotide excision repair genes in lung cancer: a case-control analysis.Carcinogenesis, vol. 21, no. 8, Aug. 2000, pp. 1527–30.
Cheng L, Spitz MR, Hong WK, Wei Q. Reduced expression levels of nucleotide excision repair genes in lung cancer: a case-control analysis. Carcinogenesis. 2000 Aug;21(8):1527–1530.
Journal cover image

Published In

Carcinogenesis

ISSN

0143-3334

Publication Date

August 2000

Volume

21

Issue

8

Start / End Page

1527 / 1530

Location

England

Related Subject Headings

  • Transcription Factors
  • Risk Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regression Analysis
  • Proteins
  • Protein Biosynthesis
  • Poly-ADP-Ribose Binding Proteins
  • Pilot Projects
  • Phytohemagglutinins
  • Oncology & Carcinogenesis