Combined effect of chemopreventive agent N-(4-hydroxyphenyl) retinamide (4-HPR) and gamma-radiation on bladder cancer cell lines.

Journal Article (Journal Article)

The incidence of bladder cancer has increased in the United States during the past 50 years, consistent with increased exposure to bladder carcinogens in the environment and tobacco use. Although N-(4-hydroxyphenyl) retinamide (4-HPR), a retinoid derivative, has been used as a chemopreventive agent of bladder cancer in clinical trials, little is known about its mechanisms of action against bladder cancer cells. Previous studies suggest this chemopreventive agent may inhibit tumor growth by inducing apoptosis. To further investigate this putative effect, we examined the effect of 4-HPR and gamma-radiation and their combined effects in three selected bladder cancer cell lines. Indeed, 4-HPR induced apoptosis in these cell lines in a dose-dependent manner. A 2.5 microM dose of 4-HPR and 50 rad of gamma-irradiation induced about 10% increase in apoptotic cells, respectively. However, this low dose 4-HPR combined with low dose gamma-irradiation had a synergistic effect on apoptosis, in which apoptotic cells increased by more than 30%. The findings have potential clinical implications and warrant further investigations both in vitro and in vivo in bladder cancer.

Full Text

Duke Authors

Cited Authors

  • Zou, C; Wang, L; Liebert, M; Grossman, HB; Lotan, R; Wei, Q

Published Date

  • November 1998

Published In

Volume / Issue

  • 13 / 5

Start / End Page

  • 1037 - 1041

PubMed ID

  • 9772297

International Standard Serial Number (ISSN)

  • 1019-6439

Digital Object Identifier (DOI)

  • 10.3892/ijo.13.5.1037


  • eng

Conference Location

  • Greece