Lipopolysaccharide and soluble CD14 in cord blood plasma are associated with prematurity and chorioamnionitis.

Journal Article (Journal Article)

BACKGROUND: Lipopolysaccharide (LPS), an endotoxin of Gram-negative bacteria, causes preterm birth in animals and has been implicated as a factor triggering preterm labor and systemic complications in humans. Little is known regarding LPS in the cord blood (CB) of term and preterm infants and its association with maternal and fetal characteristics. METHODS: CB was obtained from term (n = 15) and preterm infants (n = 76) after delivery. Plasma levels of LPS, C-reactive protein (CRP), and soluble CD14 (sCD14) were measured using commercially available kits (limulus amebocyte lysate and enzyme-linked immunosorbent assay). Four linear regression models were created in order to identify independent variables that predict plasma LPS levels. RESULTS: The analyte levels were significantly higher in preterm vs. term infant CB: LPS (24.48 vs. 1 pg/ml; P = 0.0009), CRP (87.9 vs. 47 ng/ml; P = 0.01), and sCD14 (0.32 vs.0.35 µg/ml; P = 0.013). There was a (significant) positive correlation between CB LPS levels and gestational age, birth weight, CRP levels, sCD14 levels, and association with both clinical and histological chorioamnionitis. CONCLUSION: Our data suggest that LPS is associated with preterm labor and inflammation (CRP elevation and chorioamnionitis). These findings may be relevant to the understanding of the role of LPS in prematurity and its role in preterm morbidities.

Full Text

Duke Authors

Cited Authors

  • Martinez-Lopez, DG; Funderburg, NT; Cerissi, A; Rifaie, R; Aviles-Medina, L; Llorens-Bonilla, BJ; Sleasman, J; Luciano, AA

Published Date

  • January 2014

Published In

Volume / Issue

  • 75 / 1-1

Start / End Page

  • 67 - 74

PubMed ID

  • 24135785

Pubmed Central ID

  • PMC4116808

Electronic International Standard Serial Number (EISSN)

  • 1530-0447

Digital Object Identifier (DOI)

  • 10.1038/pr.2013.182


  • eng

Conference Location

  • United States