IFNgamma primes macrophages for inflammatory activation by high molecular weight hyaluronan.

Journal Article (Journal Article)

The objective was to assess outcomes of IFNgamma-priming upon macrophage activation by the synovial macromolecule high molecular weight hyaluronan [HMW-HA] in the context of rheumatoid arthritis inflammation. Human macrophages primed by IFNgamma and activated by HMW-HA were evaluated for cytokine secretion by ELISA and Milliplex assay and activation profiles by nuclear transcription factor EIA. IFNgamma-primed, HMW-HA-activated macrophages produced elevated levels of TNF and secreted the TH1 cytokine IL-12p70, while IFNgamma suppressed HMW-HA-induced secretion of the regulatory cytokine IL-10 and activation of the transcription factor c-Jun. IFNgamma modulates the HMW-HA-induced cytokine response profile promoting macrophage activation and inflammatory TH1 cytokine secretion.

Full Text

Duke Authors

Cited Authors

  • Wallet, MA; Wallet, SM; Guiulfo, G; Sleasman, JW; Goodenow, MM

Published Date

  • 2010

Published In

Volume / Issue

  • 262 / 2

Start / End Page

  • 84 - 88

PubMed ID

  • 20299009

Pubmed Central ID

  • PMC2891046

Electronic International Standard Serial Number (EISSN)

  • 1090-2163

Digital Object Identifier (DOI)

  • 10.1016/j.cellimm.2010.02.013


  • eng

Conference Location

  • Netherlands