T lymphocyte depletion of human peripheral blood and bone marrow using monoclonal antibodies and magnetic microspheres.

Published

Journal Article

It has previously been demonstrated that graft-versus-host disease can be overcome in patients receiving HLA-mismatched bone marrow transplants by prior in vitro depletion of T lymphocytes from the marrow. In this report we describe the use of monoclonal antibodies and magnetic microspheres for the depletion of T cells from peripheral blood and bone marrow. The target cells are sensitized with antibodies directed against the CD2, CD3, CD4 and/or CD8 cell surface antigens, captured by magnetic beads coated with sheep anti-mouse IgG antibody and collected by placing the cell suspension in a magnetic field. This simple, rapid procedure results in the efficient removal of T cells from peripheral blood and from bone marrow without affecting the colony-forming potential of normal hematopoietic stem cells. The procedure is capable of being scaled up for the treatment of larger volumes of marrow that are required for clinical transplantation.

Full Text

Duke Authors

Cited Authors

  • Gee, AP; Lee, C; Sleasman, JW; Madden, M; Ugelstad, J; Barrett, DJ

Published Date

  • August 1987

Published In

Volume / Issue

  • 2 / 2

Start / End Page

  • 155 - 163

PubMed ID

  • 2901878

Pubmed Central ID

  • 2901878

International Standard Serial Number (ISSN)

  • 0268-3369

Language

  • eng

Conference Location

  • England