Whole-mount in situ hybridization on murine skeletogenic tissues

Journal Article

Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction. © 2014 Springer Science+Business Media, LLC.

Full Text

Duke Authors

Cited Authors

  • Rutkowsky, T; Sharma, D; Hilton, MJ

Published Date

  • 2014

Published In

Volume / Issue

  • 1130 /

Start / End Page

  • 193 - 201

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-989-5_14